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Showing: 3961 - 3980 of 9036 results

  1. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

    Plasmid
    #127343
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.
    Insert
    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mutation
    Base pairs 1-1554 of transgene are codon optimize…
    Promoter
    None
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    127343-plasmid-map-sequence-id-246564

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  2. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

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  3. pTXBI-mclover-YTH-IDR2

    Plasmid
    #177122
    Purpose
    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH and IDR2 domains only (IDR1 deletion)
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177122-plasmid-map-sequence-id-348676

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  8. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  11. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Expression
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  12. pMOTag2T

    Plasmid
    #26295
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Trypanosome in-situ tagging vector
    Tags
    Ty-1
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2010
    Availability
    Academic Institutions and Nonprofits only
    26295-plasmid-map-sequence-id-12380

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  13. pMOTag2H

    Plasmid
    #26296
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Trypanosome in-situ tagging vector
    Tags
    Triple HA
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2010
    Availability
    Academic Institutions and Nonprofits only
    26296-plasmid-map-sequence-id-12381

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  14. pMOTag5H

    Plasmid
    #26299
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Trypanosome in-situ tagging vector
    Tags
    triple HA
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2010
    Availability
    Academic Institutions and Nonprofits only

  15. pMOTag53M

    Plasmid
    #26297
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Trypanosome in-situ tagging vector
    Tags
    triple cMyc
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2010
    Availability
    Academic Institutions and Nonprofits only
    26297-plasmid-map-sequence-id-12382

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  16. ZM2

    Plasmid
    #32784
    Depositor
    George Cross
    Insert
    T7rnap
    Use
    Cre/Lox; Floxed targeting vector for trypanosome
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2011
    Availability
    Academic Institutions and Nonprofits only

  17. pENTR4-SB1 (w789-3)

    Plasmid
    #26956
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    SB1
    Expression
    Mutation
    Promoter
    Available since
    April 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    26956-plasmid-map-sequence-id-12766

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  18. pLEW100v5

    Plasmid
    #24011
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24011-plasmid-map-sequence-id-11282

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  19. pENTR4-SNAPf (w878-1)

    Plasmid
    #29652
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    SNAPf
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29652-plasmid-map-sequence-id-220455

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  20. pENTR4-CLIPf (w877-2)

    Plasmid
    #29650
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    CLIPf
    Expression
    Mutation
    Promoter
    Available since
    July 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29650-plasmid-map-sequence-id-13765

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Showing: 3961 - 3980 of 9036 results