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Showing: 3941 - 3960 of 4003 results

  1. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

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  3. Multiplex-CRISPR-miR-200FAM-T2A-GFP

    Plasmid
    #179856
    Purpose
    to delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200b
    Depositor
    Brendan Jenkins
    Article
    Yu et al Oncogene. 2021 Oct 19. pii: 10.1038/s413
    Insert
    hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c ) (MIR141, MIR200B, MIR429, MIR200C, MIR200A Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179856-plasmid-map-sequence-id-357409

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  4. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

    Plasmid
    #73164
    Purpose
    multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells
    Depositor
    Bruce Edgar
    Article
    Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:
    Insert
    mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
    Use
    Tags
    mRFP1, GFP
    Expression
    Insect
    Mutation
    Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…
    Promoter
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    73164-plasmid-map-sequence-id-134544

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  9. pAct-Fok1:dCas9

    Plasmid
    #62211
    Purpose
    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    act5C
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62211-plasmid-map-sequence-id-101870

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  10. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62210-plasmid-map-sequence-id-101869

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  11. pCRII-Topo Grp

    Plasmid
    #45871
    Depositor
    Jeffrey Macklis
    Insert
    GRP in situ probe (Grp Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    nt 66-504 on NM_175012
    Promoter
    Available since
    June 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid
    #172915
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7f-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172915-plasmid-map-sequence-id-341221

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  13. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid
    #172918
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7c-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172918-plasmid-map-sequence-id-343805

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  14. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

    Plasmid
    #172916
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7s-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172916-plasmid-map-sequence-id-341478

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  15. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

    Plasmid
    #172917
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7b-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172917-plasmid-map-sequence-id-343804

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  16. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

    Plasmid
    #172920
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7s-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172920-plasmid-map-sequence-id-343802

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  17. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid
    #172919
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7f-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172919-plasmid-map-sequence-id-343801

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  18. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid
    #172914
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7c-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172914-plasmid-map-sequence-id-341479

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  19. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

    Plasmid
    #172913
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7b-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172913-plasmid-map-sequence-id-341198

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Showing: 3941 - 3960 of 4003 results