Addgene

We narrowed to 14,520 results for: LEA

Showing: 3941 - 3960 of 14520 results

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PcDNA-DEST47-CypB-Golgi C-term GFP

Plasmid

#36128

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pBacPak-HA-E(y)2

Plasmid

#52288

Purpose

Expresses Drosophila E(y)2

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

E(y)2 (e(y)2 Fly)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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274_pSKB3-Venus-LUMP

Plasmid

#65976

Purpose

Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Use

Tags

Venus and thrombin site (11-residue linker that h…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBM13-sd4U

Plasmid

#179691

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)

Use

Tags

Expression

Bacterial

Mutation

Q1310R, S1330I, T1380I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBDR

Plasmid

#62663

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Nucl2 C-term GFP

Plasmid

#36126

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains CypB AA 34-211

Promoter

CMV

Available since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Nucl C-term GFP

Plasmid

#36124

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

Cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 34-216 of cyclophilin B

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Del2 C-term GFP

Plasmid

#36134

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 38-216 of cyclophilin B

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pJL6

Plasmid

#184134

Purpose

For labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

CELE_VW02B12L.1 (vha-6 Nematode)

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Pvha-6 (894bp)

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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ptreCas9-mKate2ps-T1gRNA

Plasmid

#62715

Purpose

Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Del1 C-term GFP

Plasmid

#36132

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 37-216 of cyclophilin B

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
p3xFLAG-MKL1 D301-380

Plasmid

#19852

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 301 to 380

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
p2T-CMV-CDAmax-BlastR

Plasmid

#152995

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

CDA-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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p3.2mar-GFP

Plasmid

#63694

Purpose

3.2 kb marine armor plate enhancer driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

hsp70

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMGF173

Plasmid

#96999

Purpose

NLS-GFP-lacZ leu1 insertion vector

Depositor

Wesley Sundquist, Adam Frost

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lacZ

Use

Tags

NLS-GFP

Expression

Yeast

Mutation

Promoter

Pnmt1

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 T450A

Plasmid

#19843

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 T450A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Threonine 450 to Alanine

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-hB50

Plasmid

#18967

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican N-terminus (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Human brevican N-terminus aa 1-400

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Use

Tags

FLAG and NLS

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHWBF06exp

Plasmid

#130831

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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