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Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
UseTagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expre…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsMycExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Fibrillarin
Plasmid#26673DepositorInsertFibrillarin (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLQ-5S-F30-Mango IV
Plasmid#127584PurposeExpresses 5S-F30-Mango IV rRNA from a mU6 promoterDepositorInsert5S rRNA with F30 folding scaffold and Mango IV tag
UseTagsExpressionMammalianMutationPromotermodified U6Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWalium20-10XUAS-3XFLAG-dCas9-VPR
Plasmid#78897PurposeExpresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRaDepositorInsertdCas9-VPR
UseTags3xFLAGExpressionInsectMutationPromoter10XUASAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS
Plasmid#199351PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFAExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ151 - ttTi5605_MCS
Plasmid#19330DepositorArticleInsertttTi5605 targeting region
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS_nanorod_105b
Plasmid#120347Purposephagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)DepositorInsertStaple sequences for a DNA origami nanorod
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP
Plasmid#37825DepositorHas ServiceAAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1InsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-CFP
Plasmid#90103PurposeCFP control expression plasmidDepositorInsertCFP
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK123 pDESTattB_exorh:mCerulean
Plasmid#213512PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP
Plasmid#177825PurposeHost Cell Reactivation (HCR) control GFP plasmid (pMax backbone)DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3CG
Plasmid#174189PurposeExpresses PIK3CG in mammalian cellsDepositorInsertPIK3CG (PIK3CG Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SlugABE-NNG
Plasmid#214356PurposeExpresses SlugABE-NNGDepositorInsertSlugABE-NNG
UseAAVTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPR
Plasmid#42875PurposeA crRNA expression plasmid for targeting a specific sequence.DepositorInsertCRISPR-BsaI
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSOUP
Plasmid#165419PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in AgrobacteriumInsertRep A
UseHelper plasmid required for replication for all p…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF13-Fc(DAPA)-AviTag-6xHis
Plasmid#156611PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFSF13 (TNFSF13 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only