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Purposefluorescent fusion protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIKKbeta
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Alt nameIKBKB
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SpeciesH. sapiens (human)
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Entrez GeneIKBKB (a.k.a. IKK-2, IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB)
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IKK2-EGFP was a gift from Johannes A. Schmid (Addgene plasmid # 111195 ; http://n2t.net/addgene:111195 ; RRID:Addgene_111195) -
For your References section:
Signaling molecules of the NF-kappa B pathway shuttle constitutively between cytoplasm and nucleus. Birbach A, Gold P, Binder BR, Hofer E, de Martin R, Schmid JA. J Biol Chem. 2002 Mar 29;277(13):10842-51. doi: 10.1074/jbc.M112475200. Epub 2002 Jan 18. 10.1074/jbc.M112475200 PubMed 11801607