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pSB_init-Sb66

Plasmid

#159422

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb66

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA

Plasmid

#64983

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pJB045 (ZapB-mEos2)

Plasmid

#72651

Purpose

Inducible expression of ZapB-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB-mEos2

Use

Tags

mEos2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pWS1037

Plasmid

#123049

Purpose

G_ subunit

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCG-D-mSpyCatcher-His6-E

Plasmid

#87376

Purpose

An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

mSpyCatcher

Use

Synthetic Biology

Tags

His6 tag

Expression

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3

Plasmid

#106115

Purpose

Lentiviral expression of PRKRA delta3

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK and myc

Expression

Mutation

Truncation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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BPK2014-AmCas12b

Plasmid

#121951

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b

Use

Tags

10xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046940

Plasmid

#149261

Purpose

Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12

Plasmid

#106116

Purpose

Lentiviral expression of PRKRA delta 12

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

Expression

Mutation

Truncation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-DSCP-Gateway

Plasmid

#71507

Purpose

flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Mutation

Promoter

Available since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pATD

Plasmid

#141012

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HSV-TK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAF259

Plasmid

#105494

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

Bitlucopt

Use

Atc-dependent expression in c. difficile

Tags

Expression

Mutation

Promoter

Ptet

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pWS627

Plasmid

#123064

Purpose

G___ bicistronic protein

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste4-2A-Ste18 [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST(frt)(ATG_V5-APEX2_noSTOP)

Plasmid

#168207

Purpose

Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Type

Empty backbone

Use

Unspecified; Pdest gateway

Tags

V5 APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJMC-1T

Plasmid

#197785

Purpose

Level 1 Module 1T destination vector

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-1

Plasmid

#197786

Purpose

Level 1 Module 1 destination vector

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3T

Plasmid

#197789

Purpose

Level 1 Module 3T destination vector

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-2-Reverse

Plasmid

#197801

Purpose

Level 1 Module 2 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-lldR

Plasmid

#74095

Purpose

L-lactate repressor (LldR) from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

lldR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Apr)

Plasmid

#110078

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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