pGL3-DSCP-Gateway
(Plasmid
#71507)
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Purpose(Empty Backbone) flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3-Promoter
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Backbone manufacturerPromega
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Vector typeInsect Expression ; flySTARR-seq validation vector
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Tag
/ Fusion Protein
- luciferase (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- luciferase (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-DSCP-Gateway was a gift from Alexander Stark (Addgene plasmid # 71507 ; http://n2t.net/addgene:71507 ; RRID:Addgene_71507) -
For your References section:
Enhancer-core-promoter specificity separates developmental and housekeeping gene regulation. Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, Stark A. Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 2014 Dec 15. 10.1038/nature13994 PubMed 25517091