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pMRXIP GFP-Stx17 WT

Plasmid

#45909

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-E988K

Plasmid

#211580

Purpose

CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Contains E988K mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 GFP WT

Plasmid

#35411

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pNLS-TetR-GFP

Plasmid

#103838

Purpose

Flourescent marker for tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

NLS-TetR (tetR )

Use

Tags

EGFP

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-PH-PLCD1

Plasmid

#183675

Purpose

PtdIns(4,5)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLCD1 (PLCD1 Human)

Use

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 11-140

Promoter

T7

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG_GPHN.FingR-eGFP-CCR5TC

Plasmid

#46296

Depositor

Don Arnold

Article

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

GPHN.FingR-eGFP-CCR5TC (GPHN Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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Emerin pEGFP-C1 (637)

Plasmid

#61993

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Emerin (EMD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-Stx7

Plasmid

#45921

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

syntaxin 7 (STX7 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP/golgin-160

Plasmid

#180630

Purpose

Expression of WT golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160 (GOLGA3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-wt

Plasmid

#12965

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA (RHOA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA1

Plasmid

#164932

Purpose

Expresses EGFP sgRNA1 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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AICSDP-46: MYL7-mEGFP

Plasmid

#114413

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Article

Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

Insert

MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_vsfGFP9

Plasmid

#191747

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and vsfGFP-9

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-shTACC3

Plasmid

#59355

Purpose

Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-GFP

Plasmid

#86145

Purpose

Lentiviral vector expressing Cas9-GFP

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

Cas9-GFP

Use

CRISPR and Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b-GFP-CPDSalI

Plasmid

#38257

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

eGFP

Use

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pEGFPN3 (Clonetech)

Promoter

T7

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
PB-PARP1-Apex2–eGFP

Plasmid

#211555

Purpose

A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

Apex2-eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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