-
Plasmid#144463PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORA (RORA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gagust
Plasmid#196054PurposeEncodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-Geo_st
Plasmid#87700PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBPDepositorInsertGeoCas9
UseTags10xHis-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
CN1633-rAAV-hsA2-eHGT_140h-minRho-SYFP2-WPRE3-BGHpA
Plasmid#163503PurposeDirect-expressing SYFP2 AAV VirusDepositorInsertSYFP2
UseAAVTagsExpressionMutationPromoterMinRhoAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pm-iATPSnFR1.0
Plasmid#102548Purposemembrane displayed ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AbVec_IGLC
Plasmid#183703PurposeEncode human Ig lambda chain constant region. For cloning variable region and mAb expression.DepositorInsertIGLC2 (IGLC2 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG-W-Flag-Nbs1
Plasmid#89315Purposeretroviral expression of mouse Nbs1DepositorInsertNbs1 (Nbn Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRESh-GFPII-MB-Dlk1
Plasmid#108930PurposeGFP-tagged vector expressing membrane-bound Dlk1DepositorInsertDlk1 (Dlk1 Mouse)
UseTagsGFPIIExpressionMammalianMutationlacks coding sequence for the juxtamembrane motif…PromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Empty Target Control
Plasmid#127667PurposeEncodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoterDepositorInsertCRY2-HA-2A-mCherry
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab13
Plasmid#110500PurposeFluorescent Rab13DepositorInsertRab13 (RAB13 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#122600PurposeBE PACE selection, express C-intein-Cas9-ugi fusionDepositorInsertintein-dCas9-ugi fusion
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-GFP
Plasmid#51463PurposeIs a biosensor for PIP3DepositorInsertBTK (aa 1–177) (BTK Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
secretedBirA-8his
Plasmid#32408DepositorInsertBirA
UseTags8 His tagExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb
Plasmid#198292PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobodyDepositorInsertPVCpnf_pvc13-antiMouseMHCIINb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB514
Plasmid#74856PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)
Plasmid#61929PurposeExpression of hUCH37 (ISF1) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SQ171fg/pCSacB
Plasmid#69344PurposeSQ171 fast growing strain supported by pCSacB plasmidDepositorInsertpCSacB
UseTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-nlsSED1
Plasmid#178190PurposeSED1 biosensor for expression in Arabidopsis thalianaDepositorInsertSED1 biosensor
UseTagsSV40 NLSExpressionPlantMutationPromoterpUBQ10Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:∆luc:∆xbaI
Plasmid#109035PurposeMPRA v3 empty backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoternoneAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP1-S127A
Plasmid#17790DepositorInsertYes-kinase associated protein mutant S127A (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationSerine 127 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_ybbR_Tau
Plasmid#159717PurposeExpresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tagDepositorInsertMicrotubule-associated protein tau (MAPT Human)
UseTagsZZ affinity tag, ybbR tagExpressionInsectMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL.hPGK.GP91
Plasmid#30477DepositorInserthPGK/GP91
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159816PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_EV
Plasmid#183116PurposeEmpty vector control for pLenti_EF1a constructsDepositorInsertSmall multiple cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP-sgRNA-MS2
Plasmid#153457PurposeCROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffoldDepositorInsertsgRNA empty backbone
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFR-mApple
Plasmid#203773PurposeNumber and brightness analysis of EGFR oligomerizationDepositorInsertEGFR (EGFR Human)
UseTagsmAppleExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Centromere-Targeting_gRNA
Plasmid#195125PurposegRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFPDepositorInsertChr1q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0731
Plasmid#142201PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPROX1 (PROX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2747
Plasmid#143606PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNKX6-2 (NKX6-2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HERV-Kcon-RT for Split-PE (pJUL3120)
Plasmid#190107PurposeExpresses HERV-Kcon RT (human codon optimization)DepositorInsertHERV-Kcon-RT
UseTagsbpNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3x-FLAG-hnRNPF
Plasmid#21926DepositorInserthnRNPF (HNRNPF Human)
UseTags3 FlagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC47A2_STOP
Plasmid#161405PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC47A2 (SLC47A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pORI28
Plasmid#71595PurposeEC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.DepositorTypeEmpty backboneUseIntegration vector for bacteriaTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Capri(1-10)
Plasmid#165142PurposeExpresses Capri(1-10) in mammalian cellsDepositorInsertCapri(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pDONR223_DOK1_WT
Plasmid#82884PurposeGateway Donor vector containing DOK1, part of the Target Accelerator Plasmid Collection.DepositorInsertDOK1 (DOK1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-MYO5C
Plasmid#135404PurposeExpresses GFP-tagged human MYO5C in mammalian cells.DepositorInsertMYO5C (MYO5C Human)
UseTagsEGFPExpressionMammalianMutationRelative to the GenBank MYO5C cDNA sequence repor…PromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFF838
Plasmid#61457PurposeaTc-inducible SCRIBE_lacZ(ON)DepositorInsertSCRIBE_lacZ(ON)
UseSynthetic BiologyTagsExpressionMutationPromoterpTetOAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hMYC
Plasmid#20723DepositorInsertc-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-3xFLAG-V5
Plasmid#87071PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseLentiviralTags3xFLAG-V5ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
UseTagsExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FlipCherry-T2A-GFP
Plasmid#124436PurposeExpresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cellsDepositorInsertFlipCherry-T2A-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10 T23N
Plasmid#130885PurposeExpresses human RAB10 T23N mutant with N-terminal GFP tagDepositorInsertRAB10 (RAB10 Human)
UseLentiviralTagsGFPExpressionMammalianMutationT23NPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N1-HDAC6.DC (catalytic deficient)
Plasmid#36189DepositorInsertHDAC6.DC (HDAC6 Human)
UseTagsEGFPExpressionMammalianMutationH216/611APromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT3B1
Plasmid#35522PurposeMammalian expression of human DNMT3B1 with myc tagDepositorInsertDNMT3B1 (DNMT3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
Alpha 5 integrin-GFP
Plasmid#15238DepositorInsertalpha 5 integrin (ITGA5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXPR_502_sgCD4
Plasmid#158704PurposeCD4 CRISPRa controlDepositorInsertCD4 (CD4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-GFP
Plasmid#58867PurposeAAV expression of GFP under the Syn promoterDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF0093
Plasmid#143009PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer X-null replicon
Plasmid#65461Purposecontaining 1.3 units of the X-null HBV genome (subtype ayw)DepositorInsert1.3 units of the X-null HBV genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only