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p-miR-Report + WT miR-181 BS in GATA6 3UTR

Plasmid

#22794

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
p-miR-Report + MT miR-181 BS in GATA6 3UTR

Plasmid

#22795

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pMCSF-R(mB)-luc

Plasmid

#12422

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

AML1 binding site mutation (created a KpnI site i…

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-TRPS1-P2A-mCherry

Plasmid

#203851

Purpose

Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

TRPS1 (TRPS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_WT_ADRB2_LucReporter

Plasmid

#129788

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

N/A

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pNL3.1-Erg

Plasmid

#182706

Purpose

Express Erg enhancer in mammalian cells

Depositor

Robert Rowe

Article

Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

Insert

Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114099

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Inserts

Gaussia luciferase, slow burn
Volvox Channelrhodopsin 1
Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9_sgRNA NC

Plasmid

#176259

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

H840A and D10A mutations on spCas9 to inactivate …

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2-Mcl-1 mut

Plasmid

#19133

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

NFkB binding-site mutant, the sequence GGGGTCTTCC…

Promoter

Available since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Budding Yeast, Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Promoter

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-257

Plasmid

#31063

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-257 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
HNF4A_P2-164

Plasmid

#31066

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-164 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
HNF4A_P2-285

Plasmid

#31060

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)

Plasmid

#75412

Purpose

Module for the expression of the Renilla Luciferase with the silencing suppressor P19

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

Renilla / P19

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a_crRNA NC

Plasmid

#176256

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

D917A and E1006A mutations to inactivate the endo…

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-hOct4 DE-SV40-Luc

Plasmid

#26279

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Oct4 Distal Enhancer Element (DE) (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

See Depositor Comments below

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCSF-R(mA)-luc

Plasmid

#12421

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

C/EBP binding site mutation (created a KpnI site …

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pNOC_hfnCas12a-Nlux_crRNA NR1

Plasmid

#176244

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

Promoter

Ribosomal subunit

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HNF4A_P2-135

Plasmid

#31067

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-135 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p-miR-Report + WT miR-181 BS in CDX2 3UTR

Plasmid

#22792

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only