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Plasmid#201165PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_886
Plasmid#201162PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SGEP
Plasmid#111170PurposemiR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterSFFVAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ReaChR-citrine
Plasmid#50956PurposeReaChR-citrine in lentival transfer vector under human synapsin promoterDepositorInsertReaChR-citrine
UseLentiviralTagscitrineExpressionMutationPromoterhuman synapsin promoterAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX208_CMV::mCherry-myc-VAMP2
Plasmid#199716PurposeExpresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-myc-VAMP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-GCaMP5G
Plasmid#105009Purposemitochondrial calcium sensorDepositorInsertGCaMP5G
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PL552
Plasmid#68407PurposeDonor empty vector with floxed puromycin resistance gene expression cassetteDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCuo CA Rac1
Plasmid#84643Purposeconstitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.DepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationConstitutively Active, Q61LPromotercumate-inducible promoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-2
Plasmid#129727PurposeExpressing SpCas9 and sgAAVS1-2DepositorInsertSpCas9 and sgAAVS1-2
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGE014
Plasmid#108682PurposeAll-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-MCS
Plasmid#80488Purposedonor vector for AAVS1 targeting (puromycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-Foxp1
Plasmid#45720DepositorInsertFoxP1 (Foxp1 Mouse)
UseTagsExpressionMutationPromoterCMV / b-actinAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mCherry-flex-dtA
Plasmid#58536PurposeExpresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cellsDepositorInsertdtA (DIP_RS18250 )
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-EGFP
Plasmid#89875PurposeEGFP reporter vector of iTango system for AAV productionDepositorInserttetO-EGFP
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC
Plasmid#64874Purposelentiviral vector with Flag, HA tagsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-ADRP
Plasmid#87161PurposeFluorescent protein localized to lipid droplets (LDs)DepositorInsertADRP (PLIN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_TauFRET_3xKQ_wpre
Plasmid#207522PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cellsDepositorInsertTauFRET_P301L_3xKQ
UseLentiviralTagsNeonGreen and mTurquoise2ExpressionMutationP301L; K311Q; K317Q; K321QPromoterEF1aAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG
Plasmid#175503PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-GFP
Plasmid#40125DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic NAD+ biosensor
Plasmid#186787PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasmDepositorInsertcytoplasmic NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-NLS-I-PpoI
Plasmid#46963PurposeFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.DepositorInsertNLS-I-PpoI
UseTagsHAExpressionMammalianMutationPromoterCMV-TETAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
nuclear NAD+ biosensor
Plasmid#186789PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleusDepositorInsertnuclear NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCH67 (crRNA expression vector)
Plasmid#217337PurposeCROP-seq crRNA expression vector with GFP and puro markerDepositorInsertAsDR-filler-AsDR8, GFP-puro
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPY-pHTomato
Plasmid#83501PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorInsertNPY (NPY Human)
UseTagspHTomatoExpressionMammalianMutationnonePromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PTEN-shRNA-1320
Plasmid#25638DepositorInsertPTEN-shRNA-1320 (PTEN Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZR060_CROPseq-sgCtrl-CRISPRi
Plasmid#180269PurposeLentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRiDepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-eGFP
Plasmid#176861PurposeExpresses cytosolic eGFP in astrocytesDepositorInserteGFP
UseAAVTagsExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-OTp-GCaMP6s
Plasmid#192945PurposeTo drive GCaMP6s under the control of mouse oxytocin promoterDepositorInsertGCaMP6s
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e
Plasmid#138504PurposeA-to-G base editorDepositorInsertecTadA(8e)-dLbCas12a
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HIF1 alpha
Plasmid#21101DepositorInsertHYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJEP207-AAV-CMV-MCS-pA
Plasmid#78318PurposeAAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
enAsABE8e
Plasmid#138506PurposeA-to-G base editorDepositorInsertecTadA(8e)-dAsCas12a (En)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HIF1 alpha K532R
Plasmid#21102DepositorInsertHYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e
Plasmid#138500PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGW1-Myc-Sarm1
Plasmid#50707Purposeexpression of Sarm1 in mammalian cellsDepositorInsertSarm1 (Sarm1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
UseTagsEGFPExpressionMutationPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3
Plasmid#112007PurposeFor co-expression of GCaMP6s with mRuby3DepositorHas ServiceAAV9InsertGCaMP6s-P2A-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-GFP SNAP25
Plasmid#170872PurposeMammalian expression of His-GFP SNAP25.DepositorInsertSNAP 25 (Snap25 Rat)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG vGlut1 mCherry
Plasmid#105010PurposeExcitatory presynaptic markerDepositorInsertSLC17A7
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P13
Plasmid#120024PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1.2
Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorInsertR-GECO1.2
UseTagsExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-sypHy-RGECO
Plasmid#84078PurposeCombined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosisDepositorInsertsypHy-RGECO
UseTagsExpressionMammalianMutationPromoterhSyn1Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_neomycin
Plasmid#65307PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseLentiviralTagsKDEL motif and Signal SequenceExpressionMutationPromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-TA-ERP2
Plasmid#80477PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-SNAP25
Plasmid#168031PurposeExpresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cellsDepositorInserteCFP-SNAP25(A141-G206)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:mNeonGreen-Rab5a
Plasmid#192355PurposezfRabaa tagged with mNeonGreen, expressed under the UAS promoterDepositorInsertRab5a (rab5aa Zebrafish)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-ShadowY-kCAAX
Plasmid#115564Purposedark ITRACK acceptor construct for dual color imagingDepositorInsertShadowY with KRas membrane targeting domain
UseTagsShadowYExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #1
Plasmid#44594DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29R
Plasmid#17602PurposeMammalian expression of HA tagged ubiquitin K29RDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29R; V5 deletedPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only