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Showing: 9601 - 9650 of 148090 results

  1. mDsRed-Golgi-7

    Plasmid
    #55832
    Purpose
    Localization: Golgi, Excitation: 557, Emission: 585
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    mDsRed
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pD649-HAsp-LRFN3-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156903
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    LRFN3 (LRFN3 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156903-plasmid-map-sequence-id-401024

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  14. TFORF0015

    Plasmid
    #141475
    Purpose
    Lentiviral vector for overexpressing the DRAP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DRAP1 (DRAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141475-plasmid-map-sequence-id-276561

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  18. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  19. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid
    #159916
    Purpose
    Mutagenesis of Penk
    Depositor
    Larry Zweifel
    Insert
    Penk (Penk Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159916-plasmid-map-sequence-id-317327

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  21. TFORF0282

    Plasmid
    #141561
    Purpose
    Lentiviral vector for overexpressing the MEF2C transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MEF2C (MEF2C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141561-plasmid-map-sequence-id-276819

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  32. 6xO/S luc

    Plasmid
    #69445
    Purpose
    detection/assessment of OCT4 and SOX2 transcription factor activity
    Depositor
    Lisa Dailey
    Insert
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    69445-plasmid-map-sequence-id-259015

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  38. pGreenII 0000 (No LUC)

    Plasmid
    #44465
    Depositor
    Roger Hellens
    Type
    Empty backbone
    Use
    Plant transformation
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    44465-plasmid-map-sequence-id-335235

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  44. pBABE puro EGFP

    Plasmid
    #128041
    Purpose
    Retroviral vector with N terminal EGFP
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128041-plasmid-map-sequence-id-254994

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  45. pSRE SSG

    Plasmid
    #127692
    Purpose
    Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.
    Depositor
    Justin English, Bryan Roth
    Article
    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306
    Insert
    Sindbis Structural Genome
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127692-plasmid-map-sequence-id-392665

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  49. pAAV-CAG-H2BmGreenLantern

    Plasmid
    #177332
    Purpose
    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.
    Depositor
    Pantelis Tsoulfas, Gregory Petsko, Murray Blackmore
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B (Hist1h2bq Rat)
    Use
    AAV
    Tags
    mGreenLantern
    Expression
    Mammalian
    Mutation
    This fusion protein was optimized to the Human co…
    Promoter
    CAG
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177332-plasmid-map-sequence-id-353323

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Showing: 9601 - 9650 of 148090 results