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Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only
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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE
Plasmid#138148PurposeExpresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag p21 T145A
Plasmid#16241DepositorInsertp21 (CDKN1A Human)
UseTagsFlagExpressionMammalianMutationT145APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBMCS-SortA
Plasmid#21931DepositorInsertsortase (srtA Staphylococcus aureus (strain N315))
UseTagsGB1 and His-tagExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-Renilla cDNA
Plasmid#29783DepositorInsertRenilla
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus
Plasmid#25734PurposeLentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
PB-CA-rtTA Adv
Plasmid#20910PurposepiggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoterDepositorInsertrtTA-ON-Adv
UsepiggybacTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSQT1313
Plasmid#53370Purposemultiplex gRNA expression plasmidDepositorInsertNone
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQLinkG2
Plasmid#13671DepositorInsertmulti cloning site
UseCo-expressionTagsGST, TEV, and attB1ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VAPB
Plasmid#108126PurposeER markerDepositorInsertmCherry:VAPB (VAPB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry
Plasmid#102245PurposeLentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.DepositorInsertdCas9-VPR-mCherry
UseCRISPR and LentiviralTagsNLS-VPR-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_5-HT1.0
Plasmid#140552PurposeExpresses the 5-HT sensor GRAB_5-HT1.0 in neuronsDepositorHas ServiceAAV9Insert5-HT sensor GRAB_5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG
Plasmid#85225PurposeExpresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsUseAAV and Cre/LoxTagsExpressionMutationPromoterhSynAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
mApple-N1
Plasmid#54567PurposeLocalization: N1 Cloning Vector, Excitation: 568, Emission: 592DepositorTypeEmpty backboneUseTagsmAppleExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP hsp70 K71E
Plasmid#15216DepositorInserthsp70 K71E (HSPA1A Human)
UseTagsEGFPExpressionMammalianMutationK71E, ATPase-deficient mutant.PromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBT277
Plasmid#122608Purposeexpress evoCDA1-BE4max in mammalian cellsDepositorInsertevoCDA1-BE4max
UseTagsExpressionMammalianMutationF23S A123V I195FPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG148 FRB-NFAST
Plasmid#130812PurposeExpresses FRB-NFAST in mammalian cellsDepositorInsertFRB-NFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dyn2-pmCherryN1
Plasmid#27689DepositorInsertDynamin 2 (DNM2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only