Addgene

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pRU61

Plasmid

#167692

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23

Plasmid

#106117

Purpose

Lentiviral expression of PRKRA delta23

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and myc

Expression

Mutation

AA 1-127

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb44

Plasmid

#159421

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb44

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIB CMV/TO DEST (w320-1)

Plasmid

#17400

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046910

Plasmid

#145672

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ykgMO-IGS-LuxCDABE

Plasmid

#207232

Purpose

Calprotectin sensing promoter with luciferase reporter

Depositor

Arthur Prindle

Article

Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

ykgMO-IGS

Use

Luciferase and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWB201

Plasmid

#68592

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046916

Plasmid

#145627

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pWS484

Plasmid

#123032

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (2x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS483

Plasmid

#123031

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (3x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS482

Plasmid

#123030

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (4x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK151

Plasmid

#183127

Purpose

BTK Type 4 reverse promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse CP25 promoter and forward rpoC terminator for dsRNA expression

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB327

Plasmid

#68655

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD2+E-Luc

Plasmid

#170873

Purpose

Phytobrick - E-Luc Luciferase CDS

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Mutation

c.375C>T to remove BbsI site

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCD017

Plasmid

#177322

Purpose

dCas9 expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBT005

Plasmid

#177323

Purpose

MCP-SoxS (R93A) expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Insert

SoxS

Use

Tags

MCP (MS2 Coat Protein)

Expression

Mutation

R93A

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLBH546_Tet-Cas14b1Locus

Plasmid

#112503

Purpose

Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-P5P4

Plasmid

#141064

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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