-
Plasmid#52385PurposeAedes aegypti NPY-like receptor AAE007924B (NPYLR1C) full-length clone in mammalian expression vectorDepositorInsertNPYLR1C
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2 short
Plasmid#51596Purposeexpresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)DepositorInsertRem2 (Rem2 Rat)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 R236A/L263A
Plasmid#51589Purposeexpresses RNAiR myc-tagged Rem2 non-Ca channel interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationR236A and L263A; silent mutations at shRNA 1 and …PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S69A/S334A
Plasmid#51588Purposeexpresses RNAiR myc-tagged Rem2 non-14-3-3 interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationS69A and S334A; silent mutations at shRNA 1 and 2…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
UAS-zw4 (egghead) ORF
Plasmid#38764DepositorInsertzw4 (egh Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCaShs-egghead (zw4)
Plasmid#37400DepositorInsertegghead (egh Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmTgfa UTR
Plasmid#31256DepositorInsertTgfa (Tgfa Mouse)
UseCdna for in situ hybridizationTagsExpressionMutationExon 6 of Tgfa with 3'UTRPromoterAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
ninaE-norpA
Plasmid#26854DepositorInsertno receptor potential A (norpA Fly)
UseTagsExpressionInsectMutationV963APromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
ninaE-stops m3-myc
Plasmid#26855DepositorInsertSlow Termination of Phototransduction (stops Fly)
UseTagsmycExpressionInsectMutationAmino acids 296-299 (SLRH in wild-type) are mutat…PromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWJPrP38
Plasmid#22110DepositorInsertPrP genomic vector with cloning sites
UseConstruction of targeting vectorTagsExpressionMutation2.2 Kb EcoR1 fragement isolated from pWJPrP34 and…PromoterAvailable sinceOct. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-rB50
Plasmid#18957DepositorInsertBrevican N-terminus (Bcan Rat)
UseTagsExpressionMammalianMutationBrevican N-terminus, aminoacids 1-395PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ38 (miR-1006 seed mutant perfect site)
Plasmid#17771DepositorInsertmiR-1006 seed mutant perfect site
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ39 (miR-1003 seed mutant perfect site)
Plasmid#17772DepositorInsertmiR-1003 seed mutant perfect site
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ45 (miR-1003 5' splice site mut)
Plasmid#17775DepositorInsertdme-miR-1003 minigene 5' splice mut
UseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutation5' splice site mut + compsensatory stemPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOX + JShal XR
Plasmid#16148DepositorUseXenopus oocyte expressionTagsExpressionMutationN/APromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE Rad 39-278
Plasmid#11225DepositorInsertRad (RRAD Human)
UseRetroviralTagsExpressionMammalianMutationN39/C278: deletion of amino acids 1-39 and 279-308PromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE Rad 39-297
Plasmid#11226DepositorInsertRad (RRAD Human)
UseRetroviralTagsExpressionMammalianMutationN39/C297: deletion of amino acids 1-39 and 298-308PromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE Rad C297
Plasmid#11217DepositorInsertRad (RRAD Human)
UseRetroviralTagsExpressionMammalianMutationC297: deletion of amino acids 298-308PromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEF-mRuby3
Plasmid#179730PurposeMammalian expression of mRuby3 from the EF1 promoterDepositorInsertmRuby3
UseTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE8e
Plasmid#138489PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSpCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-KRAB
Plasmid#191314PurposepiggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFPDepositorInsertsKRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP
Plasmid#157733Purposemammalian expression of untagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
NG-ABE8e
Plasmid#138491PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSpCas9 (NG)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FAM134C-GFP
Plasmid#201927PurposeFor lentiviral transduction of FAM134C-GFPDepositorInsertFAM134C (RETREG3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybGam-WPRE
Plasmid#207662PurposeControl for CybSEP2DepositorInsertGamillus
UseAAVTagsExpressionMutationHistidine 86 and 159 to alanines, inserted gamill…PromoterhSynapsinAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag Runx1 FL (P#1790)
Plasmid#14585DepositorInsertRunx1 (Runx1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagA shRNA #1
Plasmid#30319DepositorInsertRagA shRNA (RRAGA Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagA shRNA #2
Plasmid#30320DepositorInsertRagA shRNA (RRAGA Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagC shRNA #2
Plasmid#26630DepositorInsertRagC shRNA (RRAGC Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagC shRNA #1
Plasmid#26629DepositorInsertRagC shRNA (RRAGC Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
-
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-RAMP4
Plasmid#201929PurposePiggyBac Vector expressing KEIMA-RAMP4DepositorInsertRAMP4 (SERP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TEX264-GFP
Plasmid#201925PurposeFor lentiviral transduction of TEX264-GFPDepositorInsertTEX264 (TEX264 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TEX264ΔLIR-GFP
Plasmid#201926PurposeFor lentiviral transduction of TEX264ΔLIR-GFPDepositorInsertTEX264 (TEX264 Human)
UseLentiviralTagsExpressionMutationF273APromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shSmad4
Plasmid#37046DepositorInsertSmad4 (SMAD4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERP2
Plasmid#80478PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-ReaChR-citrine
Plasmid#50954PurposeReaChR-citrine in AAV2 vector under human synapsin promoterDepositorInsertReaChR-citrine
UseAAVTagscitrineExpressionMutationPromotertruncated human Synapsin promoterAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationKO: all lysines mutated to argininesPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
Plasmid#222994PurposeMammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNADepositorInsertHuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry
Plasmid#166599PurposeEncodes Cre-dependent hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_887
Plasmid#201164PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only