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pSCMV-H2B-FT-Fast

Plasmid

#157669

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Fast-FT

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSCMV-H2B-FT-Medium

Plasmid

#157670

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Medium-FT

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCMV-H2B-FT-Slow

Plasmid

#157671

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Slow-FT

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Mutation

Promoter

MSCV

Availability

Academic Institutions and Nonprofits only

Enlarge
MSI2-DCD

Plasmid

#166968

Purpose

Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-DCD

Use

Retroviral

Tags

MSI2-DCD

Expression

Mammalian

Mutation

N495S

Promoter

MSCV

Availability

Academic Institutions and Nonprofits only

Enlarge
HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Mutation

Promoter

MSCV

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mScarlet- hMeikin(1-332)-mNeonGreen

Plasmid

#174714

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

1-332

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen

Plasmid

#174715

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutations

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

1-332, E151R, R154E

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen

Plasmid

#174716

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Rec8 fused to human Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Rec8 (REC8 Human)

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

hRec8: 297-506; hMeikin: 1-332, E151R, R154E

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mScarlet- hRec8(297-506)-mNeonGreen

Plasmid

#174717

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Rec8

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Rec8 (REC8 Human)

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

297-506

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-hp100

Plasmid

#174734

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

P100 (NFKB2 Human)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-hp100 SS/AA

Plasmid

#174735

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

p100 SS/AA (NFKB2 Human)

Use

Retroviral

Tags

Expression

Mutation

SS/AA

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-mTRAF2

Plasmid

#174736

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

TRAF2 (Traf2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-myc-BioID-C

Plasmid

#174737

Purpose

Overexpresion

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLXSN(GFP)-myc-BioID-hp100

Plasmid

#174738

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Insert

P100 (NFKB2 Human)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGR1-HA-mTak1

Plasmid

#174740

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

Tak1 (Nr2c2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIGR1-HA-mTBK1

Plasmid

#174741

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

TBK1 (Tbk1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIChp-hOtub1

Plasmid

#174743

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Zhou et al Nat Immunol. 2019 Jul;20(7):879-889. doi

Insert

OTUB1 (OTUB1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IBU-PFAS-FLAG

Plasmid

#168226

Purpose

Expresses human PFAS-3xFLAG fusion in mammalian cells

Depositor

Noboru Mizushima

Article

Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

Insert

PFAS (PFAS Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIR-bsr

Plasmid

#176507

Purpose

Self-inactivating retrovirus vector containing a Blasticidin S-resistance gene

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2021 Dec 6;6(1):bpa

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge