H2B-mScarlet- hRec8(297-506)-mNeonGreen
(Plasmid #174717)

• Purpose: Stable expression of fluorescent Separase cleavage sensor with human Rec8
• Depositing Lab: Iain Cheeseman
• Publication: Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8. doi: 10.1016/j.devcel.2021.06.019. Epub 2021 Jul 30.

Backbone

• Vector backbone: pBABE-Blast
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rec8
• Species: H. sapiens (human)
• Mutation: 297-506
• Entrez Gene: REC8 (a.k.a. HR21spB, REC8L1, Rec8p)
• Tags / Fusion Proteins:
  • H2B-mScarlet (N terminal on insert)
  • mNeonGreen (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pNM937.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pNM937 . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint