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Plasmid#145370PurposeGateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tagDepositorTypeEmpty backboneUseTagsHis and mCherryExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only
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pJC225
Plasmid#176883PurposeMoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning systemDepositorTypeEmpty backboneUseCloningTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPTK002-2-pGAP
Plasmid#84961PurposeType 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoterDepositorInsertGlyceraldehyde-3-phosphate dehydrogenase promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1805 c to g)PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRU323
Plasmid#167690PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH645
Plasmid#172169PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationInserted amber codon immediately after initiator …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-GFP
Plasmid#133009PurposepFA carrying a sfGFP geneDepositorInsertsfGFP
UseTags6xhisExpressionBacterialMutationPromotertetRAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2_163
Plasmid#199095PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046768
Plasmid#145763PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCBC-DT1T2.2
Plasmid#134752PurposePCR template for sgRNA cloningDepositorInsertsgRNA-AtU6_29p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT1T2.2
Plasmid#134751PurposePCR template for sgRNA cloningDepositorInsertsgRNA-TaU3p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LoriT
Plasmid#163691PurposeModified L4440 containing now CamR and oriT for conjugationDepositorInsertoriT
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB329
Plasmid#68657PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046754
Plasmid#145647PurposeBacterial Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMpGWB129
Plasmid#68583PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1
Plasmid#106114PurposeLentiviral expression of PRKRA delta1DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsDDK (FLAG) and mycExpressionMutationAA 100-313PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRU319
Plasmid#167686PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G02
Plasmid#67136PurposeGateway destination vector for cell-free expression with C-term 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRU292
Plasmid#167687PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPTK003-2-pENO1
Plasmid#84962PurposeType 2, pENO1, Enolase 1 promoterDepositorInsertEnolase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (2350 g to c) and BsmBI site re…PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only