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pMP71-HLA-B0702-His

Plasmid

#104850

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)

Plasmid

#139447

Purpose

Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-p53DD

Plasmid

#196582

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Use

Tags

Expression

Mammalian

Mutation

Δ40-903

Promoter

CMV

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-HLA-B0702-His

Plasmid

#104849

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Entry vector for gateway system

Tags

his

Expression

Mutation

Promoter

none

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-Actin5c

Plasmid

#15312

Depositor

Ron Vale

Article

Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

Insert

Actin5c (Act5C Fly)

Use

Tags

EGFP and His

Expression

Insect

Mutation

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pCEP4-myc-CXCR4

Plasmid

#98946

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

Plasmid

#84243

Purpose

Expresses ABA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sa dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Article

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Tags

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CXCR4-VN

Plasmid

#98964

Purpose

FLAG-tagged human CXCR4 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

FLAG (dykdddd) epitope tag, Signal/leader sequenc…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-VC

Plasmid

#98967

Purpose

myc-tagged human CXCR4 fused to C-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLCO3A1

Plasmid

#132293

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO3A1 (SLCO3A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Moe-pDONR221

Plasmid

#124239

Purpose

Gateway entry clone of Drosophila Moesin cDNA encoding the D isoform.

Depositor

Peter Vilmos

Article

Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

Insert

Drosophila Moesin (Moe Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-LacI

Plasmid

#103814

Purpose

BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161Y

Plasmid

#98951

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161Y: enhanced CXCL12 binding and signaling

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161H

Plasmid

#98952

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161H: enhanced CXCL12 binding and signaling

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-H79Q

Plasmid

#98953

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant H79Q

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

H79Q: enhanced CXCL12 binding and signaling

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-HA-PHLPP1-delta-PDZ

Plasmid

#84825

Purpose

Mammalian expression of a HA tagged, human PHLPP1 containing a deletion of the PDZ binding motif

Depositor

Alexandra Newton

Article

Reyes et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

phlpp1 (PHLPP1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Deletion of amino acids 1714-1717

Promoter

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 4-178

Plasmid

#108284

Purpose

Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 179-199

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 1-143

Plasmid

#108286

Purpose

Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-143

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP1B 4-199

Plasmid

#115325

Purpose

Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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