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pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Use

Tags

pr-mEosFP

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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TGMP.shKras.1422

Plasmid

#59913

Purpose

Tet on shKras for conditional Kras knockdown

Depositor

Tyler Jacks

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

Kras (Kras Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Gdf3

Plasmid

#13358

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

growth differentiation factor 3 (Gdf3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLNCX1 HA Akt1

Plasmid

#15990

Depositor

Morris Birnbaum

Article

Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
-2.5Kb MMP10

Plasmid

#53973

Purpose

Luciferase reporter for mouse MMP10 2.5kb promoter

Depositor

Michael Chin

Article

Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

Insert

mouse MMP10 2.5Kb promoter (Mmp10 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PCR from C57BL6/J

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

Plasmid

#111387

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
ChETA

Use

AAV

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRosa26-Acceptor (AG103)

Plasmid

#99632

Purpose

To insert the Tre-Tight or UAS ifgMosaic Donor vectors

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Use

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-4

Plasmid

#11363

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-4 (Irs4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-RapR-FAK-YM

Plasmid

#25928

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-FAK (Ptk2 Mouse, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pdsRED-Mono-N1-FoxO1

Plasmid

#34678

Depositor

Domenico Accili

Insert

FoxO1-RFP (Foxo1 Mouse)

Use

Tags

Flag and RFP

Expression

Mutation

Promoter

CMV

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
Anti-Miro2 [N384/63R]

Plasmid

#182114

Purpose

Mammalian Expression Plasmid of anti-Miro2 (Human). Derived from hybridoma N384/63.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Miro2 (Homo sapiens) recombinant mouse monoclonal antibody (RHOT2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

Plasmid

#91881

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
R-R334W-(Kin-7)

Plasmid

#28178

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Use

Tags

Expression

Mammalian

Mutation

R334W

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pCR-Flag-IKKbeta-KM

Plasmid

#15466

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta-KM (Ikbkb Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Lysine at 44 to Alanine

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
GP734

Plasmid

#164527

Purpose

Expresses Flag-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin

Use

Lentiviral

Tags

FLAG and Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-Syndapin1fl

Plasmid

#36859

Depositor

Yvonne Groemping

Insert

Syndapin1 (Pacsin1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKdm6a#2/Cre

Plasmid

#173638

Purpose

Expresses a Kdm6a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kdm6a (Kdm6a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKdm6a#1/Cre

Plasmid

#173637

Purpose

Expresses a Kdm6a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kdm6a (Kdm6a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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