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Addgene

pCR-Flag-IKKbeta-KM
(Plasmid #15466)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15466 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCR3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5044
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IkB kinase beta-KM
  • Alt name
    IKKbeta-KM
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Mutation
    changed Lysine at 44 to Alanine
  • GenBank ID
    AF026524
  • Entrez Gene
    Ikbkb (a.k.a. IKK-2, IKK-B, IKK-beta, IKK2, IKK[b], IKKbeta, NFKBIKB)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pCR3.1 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-Flag-IKKbeta-KM was a gift from Hiroyasu Nakano (Addgene plasmid # 15466 ; http://n2t.net/addgene:15466 ; RRID:Addgene_15466)
  • For your References section:

    Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Nakano H, Shindo M, Sakon S, Nishinaka S, Mihara M, Yagita H, Okumura K. Proc Natl Acad Sci U S A. 1998 Mar 31. 95(7):3537-42. 10.1073/pnas.95.7.3537 PubMed 9520401