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3xDBE_RE:NLuc

Plasmid

#178318

Purpose

FOXO NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

FOXO NLuc Reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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FU- dio Teal-gephyrin - W

Plasmid

#73918

Purpose

To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Teal-gephyrin

Use

Lentiviral

Tags

Teal

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Martin Reijns, Andrew Jackson

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184S

Plasmid

#106198

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Tags

Expression

Mutation

GltI: A184S

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-3XMyc-mTrim25

Plasmid

#51394

Depositor

Narry Kim

Article

Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

Insert

Trim25 (Trim25 Mouse)

Use

Lentiviral

Tags

3XMyc

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Use

Tags

NubI

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKate2-B_UTR

Plasmid

#68480

Purpose

Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKate2-B_UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-mTurquoise2

Plasmid

#108235

Purpose

Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter - mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

codon optimized for experession in P. fluorescens

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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MSP1-bio

Plasmid

#47709

Purpose

Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MSP1 (MSP1 Synthetic, Plasmodium falciparum)

Use

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-OR51E1

Plasmid

#22323

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

OR51E1 (OR51E1 Human)

Use

Tags

Rho

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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Cerulean-2xFv-hRIPK3

Plasmid

#106079

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

Cerulean-2xFv-hRIPK3 (RIPK3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only
pCB19

Plasmid

#194757

Purpose

EGFP-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

EGFP-Tag

Expression

Mutation

none

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCB20

Plasmid

#194758

Purpose

ALFA-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

ALFA-Tag

Expression

Mutation

none

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0730

Plasmid

#143435

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF518A (ZNF518A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG mEmerald-EB3

Plasmid

#168514

Purpose

Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

Depositor

Franck Polleux

Article

Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

Insert

EB3 (MAPRE3 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA GASTM-3-RVG-10-Lamp2b-HA

Plasmid

#71295

Purpose

Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Use

Tags

GASTM mutated glycosylation motif, HA, Lamp2 sig…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

Plasmid

#176311

Purpose

Expresses NLS-pMag-CreC60 with tagBFP marker

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

Use

Lentiviral and Synthetic Biology

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

human EF1alpha

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GluN1 (px330:2-guideCas9)

Plasmid

#169426

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEY11

Plasmid

#191017

Purpose

Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

Ultrapanneuronal

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN1 TKIT donor

Plasmid

#169427

Purpose

Donor DNA for TKIT knockin of SEP to NR1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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