-
Plasmid#114177PurposeGateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ERDepositorInserttagRFP-KDEL
UseGateway entry cloneTagsKDEL retention signal and signal peptideExpressionMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-eYFP-WPRE
Plasmid#130988PurposeAAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagseYFPExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pHW449
Plasmid#198805PurposeDirect light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.DepositorInsert15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
plRL19
Plasmid#163634PurposeL5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).DepositorInsertL5 Integrase
UseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP) and Tet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RHO-IntC-dCas9C-VPR-polyA
Plasmid#166692PurposeExpress C-terminal part of split dCas9-VPRDepositorInsertIntC-dCas9C-VPR
UseAAV and CRISPRTagsExpressionMammalianMutationPromotershort human rhodopsin promoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞RFP
Plasmid#154014PurposeVector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞GFP
Plasmid#154015PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposaseDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
plRL61
Plasmid#163633PurposeOptimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-SpCas9
Plasmid#117051PurposeIn vitro expression of S. pyogenes Cas9 from the T7 promoterDepositorInsertS. pyogenes Cas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST1374-scFv-APOBEC-UGI-GB1
Plasmid#113029PurposeExpresses scFv-rAPOBEC1-UGI in mammalian cellsDepositorInsertscFv-rAPOBEC1-UGI-GB1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRmine-eYFP-WPRE
Plasmid#130992PurposeAAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoterDepositorInsertChRmine
UseAAVTagseYFPExpressionMutationPromoterhSynAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE
Plasmid#130991PurposeAAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoterDepositorHas ServiceAAV8InsertChRmine
UseAAVTagsmScarlet-Kv2.1ExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA5a-sufCDSUB
Plasmid#195055PurposeExpresses SUF operon from B. subtilis from IPTG-inducible promoterDepositorInsertsufCDSUB
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Necl-5-ScNeo
Plasmid#170283PurposeTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInserta recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)
UseTagsmScarlet and mNeonGreenExpressionMammalianMutationPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
TC1316
Plasmid#164883PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3DepositorInsertdcas13d-ADARdd
UseTagsExpressionMammalianMutationdCas13d L560-G586 deletedPromoterCMV, T7Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NANOS3-T2A-mVenus-PuroTK
Plasmid#222903PurposeHomology directed repair template for knocking in mVenus reporter to NANOS3.DepositorInsertmVenus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402-omega
Plasmid#74797PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xCaMV35S enhancer and omegaAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV/myc/ER-αT9ib
Plasmid#110768PurposeExpresses ER intrabody against mouse and human TLR9DepositorInsertintrabody against mouse and human TLR9
UseTagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only