Addgene

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pUAS-ERK-YFP

Plasmid

#42076

Depositor

Norbert Perrimon

Article

Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

Insert

Drosophila ERK ortholog (rl Fly)

Use

Tags

YFP

Expression

Insect

Mutation

Promoter

Hsp70

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pFB2B

Plasmid

#195284

Purpose

Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Parkin-IRES-zeo

Plasmid

#61727

Purpose

Expression of human Parkin fused to mCherry

Depositor

Douglas Green, Stephen Tait

Article

Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

Insert

Parkin (PRKN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFB2C

Plasmid

#195285

Purpose

Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Hygro

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR FL CPAP RNAi resistant

Plasmid

#46390

Depositor

Pierre Gonczy

Article

Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

Insert

CPAP RNAi resistant mutant (CENPJ Human)

Use

Entry vector

Tags

Expression

Mutation

silent mutation that renders it resistant to siRN…

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only
mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Synthetic, Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin 1

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

Plasmid

#182049

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S248A/T258A

Promoter

CAG

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

Plasmid

#193719

Purpose

Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Synthetic, Human)

Use

Tags

2xHA-FRB

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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