Addgene

Showing: 3761 - 3780 of 155574 results

Results per page:

Sort results by:

pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Mutation

Promoter

CMV Promoter, T7 Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

Plasmid

#118278

Purpose

Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Insert

bPAC(WT)

Use

AAV

Tags

mCherry and myc

Expression

Mutation

Promoter

CamKII

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSFFV_CloGFP(1-10)

Plasmid

#157994

Purpose

Lentiviral vector of constitutive expression of CloGFP(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

CloGFP(1-10)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G32Q, F46L, K101R, S202L

Promoter

SFFV

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Use

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-p300

Plasmid

#23252

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TO-oNGN2

Plasmid

#198397

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2
rtTA3G

Use

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

Promoter

CAG, EF1a, and TRE3G

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B_mCherry_IRES_neo3

Plasmid

#21044

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEEK2

Plasmid

#83492

Purpose

Expression plasmid for conjugational transfer into cyanobacteria

Depositor

Pia Lindberg

Article

Miao et al Metab Eng Commun. 2017 Jul 29;5:45-53. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Strep-tag

Expression

Bacterial

Mutation

Promoter

Ptrc core

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
P307-FBP_6

Plasmid

#139697

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAY5 (mAID-EGFP-NLS piggyBac)

Plasmid

#140532

Purpose

mAID-EGFP-NLS piggyBac

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

Plasmid

#188383

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4/BDD-FVIII

Plasmid

#41035

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

B domain deleted FVIII (F8 Human)

Use

Tags

Expression

Mammalian

Mutation

B domain deletion

Promoter

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-cIAP1/pRK5

Plasmid

#27972

Depositor

Xiaolu Yang

Article

Hu et al J Biol Chem. 2003 Mar 21;278(12):10055-6

Insert

CIAP1 (BIRC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only
PBCAG-mRFP

Plasmid

#40996

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge