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Plasmid#52092PurposeRetroviral expression of all four WT CD3 subunits, co-expressed with EGFPDepositorInsertsUseRetroviralTagsF2A, IRES-EGFP, P2A, and T2AExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only
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PX551
Plasmid#60957PurposepAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.DepositorInsertSpCas9
UseAAV and CRISPRTagsHAExpressionMammalianMutationPromoterpMecp2Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-HA
Plasmid#141320PurposePlasmid to carry out IVT of RfxCas13d (human codon-optimized)DepositorInsertRfx-Cas13d
UseCRISPRTagsHAExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH69
Plasmid#173782PurposeThe pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.DepositorInsertHK022 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1328 LV EF1a-CD20 IRES-EGFP
Plasmid#201918Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD20DepositorInsertCD20
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE57
Plasmid#90402PurposeType I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterGRAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP
Plasmid#154786PurposeExpresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBEDepositorInsertAdenosine deaminse (Adar Synthetic, Fly)
UseLentiviralTagsGFP, V5, and mCherryExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GFP-h-PKD2
Plasmid#83451PurposeGFP-tagged PC2 for Eukaryotic expression (883)DepositorInsertPolycystin-2 (PKD2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TOMM20-N-10
Plasmid#55146PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sg1-sg2
Plasmid#192479PurposeAll-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1DepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
3HA-ERHBD-Hoxb8
Plasmid#212932PurposeExpresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell linesDepositorUseRetroviralTagsTriple HA tagExpressionMammalianMutationPromoterMSCV LTRAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-pcDNA3
Plasmid#18950DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralTagsExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0236
Plasmid#142489PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCN (MYCN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-mCh-Cry2WT
Plasmid#101223PurposeFUS(1-214) fused to mCh-Cry2WTDepositorInsertFUS(aa1-214) (FUS Human)
UseLentiviralTagsmCherry-Cry2ExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CN2102-rAAV-eHGT_387m-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#208133PurposeDirect-expressing SYFP2 in astrocytes AAV VirusDepositorInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE
Plasmid#180280PurposeThe CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.DepositorInsertCasMINI sgRNA (backbone) and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK123 pDESTattB_exorh:mCerulean
Plasmid#213512PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGRB2.3
Plasmid#45343DepositorTypeEmpty backboneUseC. glabrata expressionTagsExpressionMutationPromoterPGK1Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only