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Plasmid#100749PurposePart of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tagDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsFRB and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GRM2-Tango
Plasmid#66388PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGRM2 (GRM2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC13N-iCAG.copGFP
Plasmid#66578PurposeCLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENsDepositorInsertcopGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-EEA1
Plasmid#169067PurposeExpress human EEA1 in mammalian cellsDepositorInsertEEA1 (EEA1 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Mango II x 24
Plasmid#127587PurposeExpresses the mCherry-Mango II x 24 array from a TetON responsive promoterDepositorInsertmCherry-Mango II x24
UseTagsmCherryExpressionMammalianMutationPromoterpTRE Tet ONAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMax_mPlum
Plasmid#177830PurposeHost Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)DepositorInsertmPlum
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS117 His14-Avi-45xGS-anti GFP nanobody
Plasmid#199370PurposeBacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-GFP nanobody
UseTags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag
Plasmid#167269PurposeOverexpression of SNAP-tag in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-ATG5
Plasmid#13095DepositorInsertATG5 (Atg5 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA1er
Plasmid#58216PurposeRed fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertR-CEPIA1er
UseTagsmycExpressionMammalianMutationR-GECO1 E300D M305L D329N F332I E336D N346K F361W…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF0427
Plasmid#142620PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPBX1 (PBX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGuide
Plasmid#64711PurposeCloning and expression of Streptococcus pyogenes guide RNADepositorInsertStreptococcus pyogenes guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDM2p-Mdm2-YFP
Plasmid#53962PurposeExpresses YFP tagged Mdm2 from MDM2 promoterDepositorInsertMDM2 (MDM2 Human)
UseTagsEYFPExpressionMammalianMutationPromoterMDM2Available sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDmAct5C::Cas9-2A-Neo
Plasmid#176679PurposeExpression of Drosophila codon optimized spCas9 under Drosophila Act5C promoterDepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprTagsExpressionInsectMutationDrosophila codon optimizedPromoterD. melanogaster Act5CAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTripZ RB1 WT-3XFLAG
Plasmid#209070PurposeDOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tagDepositorInserthuman RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
UseLentiviralTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
UseTagsExpressionMammalianMutationA215CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
UseTagsExpressionMammalianMutationD210N, catalytically inactivePromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only