Addgene

We narrowed to 28,566 results for: uros

Showing: 9351 - 9400 of 28566 results

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-sfGFP-GSAx9-iCre-ERT2

Plasmid

#125748

Purpose

sfGFP and iCreERT2 fused by GSAx9 linker

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

sfGFP-GSAx9-iCre-ERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Prrx1

Plasmid

#84322

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Prrx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Prrx1 (Prrx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p426-SNR52p-gRNA.clr-2.Y-SUP4t

Plasmid

#89692

Purpose

gRNA for clr-2 locus

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gRNA for clr-2 locus

Use

CRISPR; Grna

Tags

No

Expression

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

Plasmid

#58522

Purpose

Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-tWnt5a

Plasmid

#169372

Purpose

Expresses a part of Chinese soft-shell turtle Wnt5a for in situ hybridization probe.

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

Insert

Wnt5a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-tWnt7b

Plasmid

#169371

Purpose

Expresses a part of Chinese soft-shell turtle Wnt7b for in situ hybridization probe.

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

Insert

Wnt7b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-Y-GECO2m

Plasmid

#105070

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Use

Tags

6x His tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBN79

Plasmid

#86717

Purpose

DamID plasmid for C. elegans

Depositor

Peter Askjaer

Article

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::Dam::MYC::Emr-1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-100u

Plasmid

#13539

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

S118W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S129N

Plasmid

#51587

Purpose

expresses RNAiR myc-tagged Rem2 non-GTP binding

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

S129N; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 L317G

Plasmid

#51586

Purpose

expresses RNAiR myc-tagged Rem2 non-CaM interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

L317G; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-mBirA272-NRX3b

Plasmid

#43921

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

BirA272-NRX3b (birA Human, Synthetic)

Use

Tags

2XFLAG

Expression

Mammalian

Mutation

See comments

Promoter

CAG

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pISH-Trpc2-probe1

Plasmid

#105473

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Cell Rep. 2014 Jul 24;8(2):583-95. doi:

Insert

Trpc2 (Trpc2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pMH184

Plasmid

#122857

Purpose

MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-modified sgRNA targeting the Arabidopsis FT promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4252811

Plasmid

#153563

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSox2-bd_mFGF_eGFP

Plasmid

#34704

Depositor

Hollis Cline

Article

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

Expression

Mutation

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
nSR100L4

Plasmid

#35174

Depositor

Benjamin Blencowe

Article

Calarco et al Cell. 2009 Sep 4;138(5):898-910.

Insert

MmnSR100

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pTorPE-Y-GECO2.2s

Plasmid

#105069

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.2s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT Pax7 N391

Plasmid

#17525

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 391 amino acids

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ1666

Plasmid

#51485

Purpose

miniMos transposable element with PuroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-mBirA272-NRX3b D137A

Plasmid

#43922

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

BirA272-NRX3b (birA Human, Synthetic)

Use

Tags

2XFLAG

Expression

Mammalian

Mutation

D137A

Promoter

CAG

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF226

Plasmid

#121667

Purpose

EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

cas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hs-norpA

Plasmid

#26858

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

no receptor potential A (norpA Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pISH-Pou2f3

Plasmid

#84321

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pou2f3 (Pou2f3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CW3SL-Myr-mCherry-Dr-TrkA

Plasmid

#127962

Purpose

Myr mCherry DrTrkA expression under CAMK promoter

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

mCherry DrTrkA

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT Pax7 34C

Plasmid

#17522

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-33

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
CJ29 (let-7 perfect site)

Plasmid

#17766

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

let-7 perfect site (let-7 Fly)

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Emx1

Plasmid

#84309

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Emx1 (Emx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Trpc2-KO TV

Plasmid

#105449

Purpose

targeting vector to generate a Trpc2 deletion mouse strain.

Depositor

Peter Mombaerts

Article

Omura et al Cell Rep. 2014 Jul 24;8(2):583-95. doi:

Insert

Trpc2-loxP-pgK-neo-pA-loxP (Trpc2 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
L1-YA

Plasmid

#15525

Depositor

Vance Lemmon

Article

Cheng et al J Neurochem. 2005 Aug . 94(4):1102-10.

Insert

L1-cytoplasmic domain-Y1176A (L1CAM Human)

Use

Tags

Expression

Yeast

Mutation

Tyrosine 1176 changed to Alanine

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/M13

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSU6-shTom40.3/CMV-eGFP

Plasmid

#89797

Purpose

Expresses shRNA against TOM40.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Use

Tags

eGFP under separate promoter

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Tshz3

Plasmid

#74362

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

eashirt zinc finger family member 3 (Tshz3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT Pax7 N33-170C

Plasmid

#17526

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 34-159 (paired domain)

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N215-273C

Plasmid

#17527

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 216-272 (homeodomain)

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Msx2

Plasmid

#84314

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx2 (Msx2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mGluR6 (200bp crit reg+SV40 bas prom)-LacZ

Plasmid

#18806

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

mGluR6 (200bp crit reg+SV40 bas prom)-LacZ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pQCXIP X2/pTER shLUC (w454-1)

Plasmid

#17491

Purpose

Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE Rad C249

Plasmid

#11215

Depositor

Ronald Kahn

Article

Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9

Insert

Rad (RRAD Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C249: deletion of amino acids 248-308

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
Cenp-B INCENP Δcen mCherry

Plasmid

#45227

Depositor

Susanne Lens, Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

vsv Cenp-B INCENP Δcen mCherry

Use

Tags

Expression

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pBS chick LH-2B [TJ#34]

Plasmid

#16271

Depositor

Thomas Jessell

Article

Liem et al Cell. 1997 Oct 3. 91(1):127-38.

Insert

LH2B (LHX9 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Plasmid

#15629

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pLKO-cfNHERF1_4

Plasmid

#195225

Purpose

shRNA to canine NHERF1.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF1 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-cfNHERF1_5

Plasmid

#195226

Purpose

shRNA to canine NHERF1.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF1 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-cfNHERF2_4

Plasmid

#195227

Purpose

shRNA to canine NHERF2.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF2 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-cfNHERF2_5

Plasmid

#195228

Purpose

shRNA to canine NHERF2.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF2 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-SOD1

Plasmid

#222568

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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