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Plasmid#182490PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-1
Plasmid#190095Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-1 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 6704-6709 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-2
Plasmid#190096Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-2 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 7122-7129 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pT7-EGFP-C1-HseIF4G1_615-654_AD
Plasmid#148518PurposeMammalian Expression of HseIF4G1_615-654DepositorInsertHseIF4G1_615-654 (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD
Plasmid#148519PurposeMammalian Expression of HseIF4G1_615-654-L640AV646ADepositorInsertHseIF4G1_615-654-L640AV646A (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD
Plasmid#148523PurposeMammalian Expression of HseIF4G1_615-654-LIVVtoAAAADepositorInsertHseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD
Plasmid#148524PurposeMammalian Expression of HseIF4G1_615-654-Y619AL624AL625ADepositorInsertHseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET21a Sa-iPGM-bio
Plasmid#179523PurposeExpresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertS. aureus iPGM bio-6His
UseTagsHis tag and biotinylation sequenceExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Mo-iPGM-bio
Plasmid#179614PurposeExpresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertM. orale iPGM bio-6His
UseTagsHis tag and biotinylation sequenceExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Hp-iPGM-bio
Plasmid#179615PurposeExpresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertH. pylori iPGM bio-6His
UseTagsHis tag and biotinylation sequenceExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP
Plasmid#178857PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFPDepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670
Plasmid#178858PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670DepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRX-C751-E3616S
Plasmid#182844Purposemutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG inductionDepositorInsertTRX (w Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-HseIF4E_AF
Plasmid#148669PurposeBacterial Expression of HseIF4EDepositorInsertHseIF4E (EIF4E Human)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence of i…PromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluJo-Y 240-2996
Plasmid#171131PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluSz 3501-4065
Plasmid#171132PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only