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Showing: 7441 - 7480 of 148587 results

  10. pLenti-IFT20-EGFP

    Plasmid
    #118032
    Purpose
    Expresses IFT20-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human IFT20 (IFT20 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118032-plasmid-map-sequence-id-230381

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  17. pBBR-Cas9ΔyqhD-HA500

    Plasmid
    #220369
    Purpose
    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440
    Depositor
    Yilan Liu
    Inserts
    SpCas9
    sgRNA for yqhD in P. putida KT2440
    Use
    CRISPR; Broad host range
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    220369-plasmid-map-sequence-id-437258

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  18. CX3C1-DuET

    Plasmid
    #213217
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CX3C1 (CX3CR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213217-plasmid-map-sequence-id-430779

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  20. pLPC-N MYC

    Plasmid
    #12540
    Depositor
    Titia de Lange
    Insert
    N MYC
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2006
    Availability
    Academic Institutions and Nonprofits only
    12540-plasmid-map-sequence-id-123590

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  24. F2RL3-Tango

    Plasmid
    #66279
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    F2RL3 (F2RL3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pRL623

    Plasmid
    #58494
    Purpose
    helper plasmid for bacterial conjugal DNA transfer
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Insert
    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    58494-plasmid-map-sequence-id-420569

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  28. pC1-SypHer3s-IMS

    Plasmid
    #108120
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
    Depositor
    Vsevolod Belousov
    Article
    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.
    Insert
    SypHer3s-IMS
    Use
    Tags
    ratiometricYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108120-plasmid-map-sequence-id-262557

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  35. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

    Plasmid
    #127362
    Purpose
    gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127362-plasmid-map-sequence-id-246307

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  36. pJH3626

    Plasmid
    #179630
    Purpose
    Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    RFP
    Expression
    Worm
    Mutation
    Promoter
    Pacr-2(s)
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    179630-plasmid-map-sequence-id-355716

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  40. ZJOM43

    Plasmid
    #133652
    Purpose
    Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPS4-DuDre (VPS4 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133652-plasmid-map-sequence-id-308131

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Showing: 7441 - 7480 of 148587 results