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Plasmid#223515Purposecogfp under control of PtacDepositorArticleInsertCoGFP
UseTagsHis tagExpressionBacterialMutationPromoterPtac and Ptac and PtetAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAND-MCS
Plasmid#223514PurposePlasmid backbone with multiple cloning site for two genes in opposing directions (convergently transcribed)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtac and PtetAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap- TagIN-HA
Plasmid#196491PurposeTo tag endogenous mouse GFAP with HADepositorInsertsHA
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-GeneTRAP
Plasmid#196492PurposeTo KO and genetrap mouse GFAP simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-GeneTRAP
Plasmid#196493PurposeTo KO and genetrap mouse VAMP2 simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-TagIN-HA
Plasmid#196494PurposeTo tag endogenous mouse VAMP2 with HADepositorInsertsHA
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-5
Plasmid#221479PurposeGateway cloning of NKX2-5 overexpressionDepositorInsertNKX2-5 (NKX2-5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MLG-HMF-LST2
Plasmid#220786PurposeExpresses N-terminal His10-Myc-FLAG-LST2 in mammalian cellsDepositorArticleInsertLST2 (ZFYVE28 Human)
UseTags10xHis-Myc-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAceBAC2-MLG-HMF-LST2
Plasmid#220787PurposeExpresses N-terminal His10-Myc-FLAG-LST2 in insect cellsDepositorArticleInsertLST2 (ZFYVE28 Human)
UseTags10xHis-Myc-FLAGExpressionInsectMutationPromoterp10Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-PLIN3
Plasmid#222420PurposeTo express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertPLIN3 (PLIN3 Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-LAP2beta
Plasmid#222416PurposeTo express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLAP2beta (Tmpo Rat)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNoRBS
Plasmid#218167PurposepET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levelsDepositorInsertShort insert
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLBD16-YFP-GW
Plasmid#209369PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis LBD16 promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCR-YFP-GW
Plasmid#209366PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis SCR promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWOL-YFP-GW
Plasmid#209365PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pADH1-YFP-GW
Plasmid#209364PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis ADH1 promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOBL-YFP-GW
Plasmid#209368PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis COBL promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAB1-YFP-GW
Plasmid#209363PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis CAB1 promoter driving expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-J1~398-Luc
Plasmid#208639Purposefor in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19DepositorInsertHepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
UseLuciferase; In vitro transcription templateTagsFirefly Luciferase (F-Luc and T7 promoterExpressionMutationnt 1~398, in frame with donstream F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only