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pAAV-CaMKIIa-JEDI-2P-WPRE

Plasmid

#179466

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-FLAG-SHLD1

Plasmid

#114115

Purpose

Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD1 (SHLD1 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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REV human OCT4 TALEN

Plasmid

#52413

Purpose

TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN

Use

TALEN

Tags

Expression

Mutation

Promoter

CAGGS

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-V5-SHLD3

Plasmid

#114124

Purpose

Expresses N-terminally tagged V5-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-eGFP-SHLD3

Plasmid

#114125

Purpose

Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Use

Tags

Expression

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FRB-MCU-Flag-TetOFF

Plasmid

#185659

Purpose

Tet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

FRB and Flag

Expression

Mammalian

Mutation

Promoter

pTight TRE

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 V908L

Plasmid

#136370

Purpose

Expresses epitope-tagged human TET3 V908L variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Mutation

V908L

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 V1089M

Plasmid

#136369

Purpose

Expresses epitope-tagged human TET3 V1089M variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2x Strep-tagII, FLAG, and HA

Expression

Mammalian

Mutation

V1089M

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 A1076T

Plasmid

#136368

Purpose

Expresses epitope-tagged human TET3 A1076C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

A1076T

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 R752C

Plasmid

#136367

Purpose

Expresses epitope-tagged human TET3 R752C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

R752C

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 F1072C

Plasmid

#136366

Purpose

Expresses epitope-tagged human TET3 F1072C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

F1072C

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Td-CGBE

Plasmid

#196599

Purpose

Mammalian expression of Td-CGBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS

Use

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N46L); S. pyogenes Cas9 (D10A)

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iCreV

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311/NLS-Cas13d-P2A-Blast

Plasmid

#212196

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

Depositor

Stacey Edwards

Article

Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

Insert

CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin

Use

CRISPR and Lentiviral

Tags

HA, NLS, and blasticidin S deaminase

Expression

Mammalian

Mutation

Promoter

EF-1α promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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px459-AMBRA1 #3

Plasmid

#172606

Purpose

Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-Puro

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 blast

Plasmid

#104997

Purpose

This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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