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RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations.

Yao S, Yuan P, Ouellette B, Zhou T, Mortrud M, Balaram P, Chatterjee S, Wang Y, Daigle TL, Tasic B, Kuang X, Gong H, Luo Q, Zeng S, Curtright A, Dhaka A, Kahan A, Gradinaru V, Chrapkiewicz R, Schnitzer M, Zeng H, Cetin A
Nat Methods. 2020 Mar 23. pii: 10.1038/s41592-020-0774-3. doi: 10.1038/s41592-020-0774-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140131pAAV-EF1a-NCreVcan be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer
140132pAAV-EF1a-CCreVcan be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer
140133pAAV-EF1a-CDreVcan be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer
140135pAAV-EF1a-iCreVcan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase
140136pAAV-EF1a-iDreVcan be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase
140137pAAV-EF1a-iFlpVcan be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

Antibodies from Article