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Showing: 9201 - 9250 of 156455 results

  10. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid
    #127392
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid and Threon…
    Promoter
    pCAG
    Available since
    July 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    127392-plasmid-map-sequence-id-250433

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  11. AAV-mDlx-jGCaMP8f-WPRE

    Plasmid
    #176753
    Purpose
    jGCaMP8f expression in GABA-ergic interneurons under the control of mDlx enhancer
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    mDlx
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176753-plasmid-map-sequence-id-349044

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  12. PX552

    Plasmid
    #60958
    Purpose
    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Inserts
    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin and U6
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60958-plasmid-map-sequence-id-382829

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  13. TPP1-bio-His

    Plasmid
    #52090
    Purpose
    Expresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TPP1 (TPP1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52090-plasmid-map-sequence-id-80366

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  16. pSBtet-Hyg

    Plasmid
    #60508
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60508-plasmid-map-sequence-id-97539

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  20. pCMV-CreEGFP

    Plasmid
    #165140
    Purpose
    Expression of Cre recombinase fused with EGFP in C-terminal
    Depositor
    Daekee Lee, David Threadgill
    Insert
    Cre recombinase
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    165140-plasmid-map-sequence-id-326583

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  24. eIF2a 2

    Plasmid
    #21808
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S52A
    Promoter
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21808-plasmid-map-sequence-id-229779

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  27. pLX-TRV2

    Plasmid
    #180516
    Purpose
    Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker
    Depositor
    Jose-Antonio Daros
    Article
    Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:
    Insert
    Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S CaMV
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    180516-plasmid-map-sequence-id-355071

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  28. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

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  30. dCas9-CBP FL

    Plasmid
    #179550
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179550-plasmid-map-sequence-id-354775

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  34. TFORF2108

    Plasmid
    #143338
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    L3MBTL4 (L3MBTL4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143338-plasmid-map-sequence-id-400322

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Showing: 9201 - 9250 of 156455 results