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Showing: 9151 - 9200 of 148090 results

  2. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Use
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

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  9. pCDNA4-myc-His-mPGC-1a

    Plasmid
    #24925
    Depositor
    Toren Finkel
    Insert
    PGC-1a (Ppargc1a Mouse)
    Use
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2010
    Availability
    Academic Institutions and Nonprofits only

  17. pHCL149

    Plasmid
    #134457
    Purpose
    CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    Para-popZ-rbs-H3H4-msfGFPN-CTM.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Para
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134457-plasmid-map-sequence-id-270548

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  18. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

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  20. EF1a_Puro_Telo_v1

    Plasmid
    #195138
    Purpose
    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction
    Depositor
    Jason Sheltzer
    Article
    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11
    Insert
    Telomere (RTEL1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195138-plasmid-map-sequence-id-383996

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  22. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  24. TFORF1332

    Plasmid
    #143790
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFAP2E (TFAP2E Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143790-plasmid-map-sequence-id-391571

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  25. TFORF2234

    Plasmid
    #143180
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA6 (GATA6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143180-plasmid-map-sequence-id-397848

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  27. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  30. pHLmMBP-3

    Plasmid
    #72345
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72345-plasmid-map-sequence-id-133752

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  33. pDsRed-attP

    Plasmid
    #51019
    Purpose
    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
    Depositor
    Jill Wildonger, Melissa Harrison, Kate O'Connor-Giles
    Article
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and None
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51019-plasmid-map-sequence-id-222558

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  36. rAAV2/cMG

    Plasmid
    #184539
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-cMG capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R, A589D; insertion o…
    Promoter
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184539-plasmid-map-sequence-id-361936

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  38. TFORF0694

    Plasmid
    #141664
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF4A (HNF4A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141664-plasmid-map-sequence-id-397916

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  39. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210706-plasmid-map-sequence-id-416266

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  40. R777-E037 Hs.DUSP3

    Plasmid
    #70321
    Purpose
    Gateway ORF clone of human DUSP3 [NM_004090.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP3 (DUSP3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70321-plasmid-map-sequence-id-125770

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  42. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Use
    Tags
    Met-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

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  43. pTCF158 Human beta-2 microglobulin

    Plasmid
    #180457
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Human beta-2 microglobulin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180457-plasmid-map-sequence-id-355801

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  44. pLVX-H3.3K27M-FLAG-BFP

    Plasmid
    #209434
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209434-plasmid-map-sequence-id-424833

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  46. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210704-plasmid-map-sequence-id-416247

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  47. pD649-HAsp-L1CAM-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157031
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    L1CAM (L1CAM Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157031-plasmid-map-sequence-id-405791

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  49. OaAEP1_C247A

    Plasmid
    #200307
    Purpose
    Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)
    Depositor
    Artur Krężel
    Article
    Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27
    Insert
    Asparaginyl endopeptidase from Oldenlandia affinis
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Cysteine 247 to Alanine
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200307-plasmid-map-sequence-id-394865

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Showing: 9151 - 9200 of 148090 results