NanoLuc reporters identify COL4A5 nonsense mutations susceptible to drug-induced stop codon readthrough.
Omachi K, Kai H, Roberge M, Miner JH
iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. eCollection 2022 Mar 18. PubMed Article
Omachi K, Kai H, Roberge M, Miner JH
iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. eCollection 2022 Mar 18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198106 | pLV-Puro_CI-hCOL4A5-Nluc | lentiviral expression of hCOL4A4 in mammalian cells |
| 198107 | pLV-Puro_CI-hCOL4A5-Nluc_C29X | lentiviral expression of hCOL4A5_C29X in mammalian cells |
| 198108 | pLV-Puro_CI-hCOL4A5-Nluc_S36X | lentiviral expression of hCOL4A4_S36X in mammalian cells |
| 198109 | pLV-Puro_CI-hCOL4A5-Nluc_R1563X | lentiviral expression of hCOL4A4_R1563Xin mammalian cells |
| 198111 | pLV-Puro_CI-hCOL4A5-Nluc_R1683X | lentiviral expression of hCOL4A4_R1683Xin mammalian cells |
| 198112 | pLV-Puro_CI-hCOL4A5-Nluc_C1684X | lentiviral expression of hCOL4A4_C1684X in mammalian cells |
| 198113 | pNLF1-C_hCOL4A5-Nluc | Express hCOL4A5 in mammalian cells |
| 198114 | pNLF1-C_hCOL4A5-Nluc_G5X | Express hCOL4A5_G5X in mammalian cells |
| 198115 | pNLF1-C_hCOL4A5-Nluc_C29X | Express hCOL4A5_C29X in mammalian cells |
| 198116 | pNLF1-C_hCOL4A5-Nluc_S36X | Express hCOL4A5_S36X in mammalian cells |
| 198117 | pNLF1-C_hCOL4A5-Nluc_E130X | Express hCOL4A5_E130X in mammalian cells |
| 198119 | pNLF1-C_hCOL4A5-Nluc_Q1016X | Express hCOL4A5_Q1016X in mammalian cells |
| 198122 | pNLF1-C_hCOL4A5-Nluc_S1632X | Express hCOL4A5_S1632X in mammalian cells |
| 198123 | pNLF1-C_hCOL4A5-Nluc_S1661X | Express hCOL4A5_S1661X in mammalian cells |
| 198124 | pNLF1-C_hCOL4A5-Nluc_R1683X | Express hCOL4A5_R1683X in mammalian cells |