pNLF1-C_hCOL4A5-Nluc_G5X
(Plasmid
#198114)
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PurposeExpress hCOL4A5_G5X in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 198114 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepNLF1-C
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Backbone manufacturerPromega
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOL4A5
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SpeciesH. sapiens (human)
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MutationG5X
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Entrez GeneCOL4A5 (a.k.a. ASLN, ATS, ATS1, CA54)
- Promoter CMV, beta-globin/IgG chimeric intron
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Tag
/ Fusion Protein
- Nluc (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNLF1-C_hCOL4A5-Nluc_G5X was a gift from Jeffrey Miner (Addgene plasmid # 198114 ; http://n2t.net/addgene:198114 ; RRID:Addgene_198114) -
For your References section:
NanoLuc reporters identify COL4A5 nonsense mutations susceptible to drug-induced stop codon readthrough. Omachi K, Kai H, Roberge M, Miner JH. iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. eCollection 2022 Mar 18. 10.1016/j.isci.2022.103891 PubMed 35243249
