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pRK5-HA-Ubiquitin-K11

Plasmid

#22901

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K11 only, all other lysines mutated to arginines

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-mGFP

Plasmid

#14757

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Has Service

Cloning Grade DNA

Insert

membrane bound form of EGFP

Use

Tags

Palmitoylation signal

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Use

Tags

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-XTbx5-GR

Plasmid

#117248

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1999 Apr;126(8):1739-51.

Insert

Tbx5-GR (tbx5-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

chimeric protein with human glucocorticoid recep…

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG536

Plasmid

#105423

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Nuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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AnkyrinB-2XHA

Plasmid

#31057

Depositor

Vann Bennett

Article

Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

Insert

Ankyrin B (ANK2 Human)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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TFORF1500

Plasmid

#143960

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RBL2 (RBL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Expression

Mutation

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2 deltaB deltaM

Plasmid

#16069

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pON.mCherry

Plasmid

#84821

Purpose

Constitutive Expression plasmid with mCherry fluorescent protein

Depositor

Howard Shuman

Article

Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tac Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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HA-VHL-pRc/CMV

Plasmid

#19999

Depositor

William Kaelin

Article

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Promoter

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-TLG1

Plasmid

#105261

Purpose

Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg1 (TLG1 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:Renilla

Plasmid

#118066

Purpose

Transcriptional unit for constitutive expression of Renilla in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GreenRenilla:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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1436 pcDNA3 Flag HA

Plasmid

#10792

Purpose

pcDNA3 vector with added Flag, HA tags

Depositor

William Sellers

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Use

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-Luc

Plasmid

#122694

Purpose

Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

Firefly luciferase

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC19-13XLexAop2-IVS-myr::GFP

Plasmid

#26224

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

myr::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Lifeact-7

Plasmid

#54602

Purpose

Localization: Actin, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Type

Empty backbone

Use

Tags

Lifeact and mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pDSHA-SARA-Myc

Plasmid

#67368

Purpose

Mammalian expression of hSara with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

Plasmid

#200401

Purpose

Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Open-loop DART VADAR sensor entry cassette

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RCAS cVax (CC#337)

Plasmid

#15136

Depositor

Connie Cepko

Article

Schulte et al Neuron. 1999 Nov . 24(3):541-53.

Insert

cVax (VAX1 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-nEF-Coff/Fon DREADD Gi-mCherry

Plasmid

#177669

Purpose

Expresses DREADD Gi only in Flp expressing cells and not in Cre expressing cells

Depositor

Karl Deisseroth

Article

Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

nEF

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3C2G

Plasmid

#171946

Purpose

Expresses PIK3C2G in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

PIK3C2G (PIK3C2G Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-SARA

Plasmid

#67473

Purpose

Mammalian expression of hSara with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMIY-mETV3-Flag

Plasmid

#34579

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

FLAG and IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
CMV-CjABE8e

Plasmid

#189929

Purpose

Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

CjABE8e

Use

Tags

Expression

Mammalian

Mutation

CjCas9 D8A

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mEGFP

Plasmid

#18696

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

monomeric EGFP

Use

Tags

Expression

Mammalian

Mutation

A206K mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pscAAV-CAG-RLuc

Plasmid

#83280

Purpose

recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

Insert

Renilla Luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pD2_miniluc_newP7

Plasmid

#174105

Purpose

adding the mini luciferase and mini promoter to the MPRA lenti construct

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Insert

mini luciferase mini-promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hfDECOR-F148A

Plasmid

#219546

Purpose

Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

hfDECOR-F148A

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Changed TadA8e Phenylalanine 148 to Alanine

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DECOR

Plasmid

#219545

Purpose

Expression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

DECOR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hfDECOR-V106G

Plasmid

#219547

Purpose

Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

hfDECOR-V106G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Changed TadA8e Valine 106 to Glycine

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CasRx gRNA targeting EZH2

Plasmid

#219655

Purpose

hU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

CasRx gRNA targeting EZH2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-eGFP-TAZ

Plasmid

#221213

Purpose

part A of TAZ-SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

FKBP-eGFP-TAZ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HyperNova/pRSETB

Plasmid

#221456

Purpose

Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-LacO-LgBiT

Plasmid

#216779

Purpose

Expresses LgBiT in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

LgBiT

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-IRF3

Plasmid

#221260

Purpose

Expression of IRF3 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Insert

IRF3 (Irf3 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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