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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3961
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecaveolin-1
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Alt nameCAV1, cav-1
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SpeciesCanis lupus familiaris
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Insert Size (bp)1313
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GenBank IDNM_001003296
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Entrez GeneCAV1
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Tag
/ Fusion Protein
- monomeric Cherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer ACC TCT ACA AAT GTG GTA TGG CTG (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAV1-mCherry was a gift from Ari Helenius (Addgene plasmid # 27705 ; http://n2t.net/addgene:27705 ; RRID:Addgene_27705) -
For your References section:
Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Hayer A, Stoeber M, Bissig C, Helenius A. Traffic. 2010 Mar . 11(3):361-82. 10.1111/j.1600-0854.2009.01023.x PubMed 20070607