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pCMycDam

Plasmid

#59218

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Mutation

Promoter

Drosophila heat-shock promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-BDNF-pHluorin

Plasmid

#187193

Purpose

To express the mouse BDNF tagged with the FP pHluorin

Depositor

Pantelis Tsoulfas

Insert

BDNF (Bdnf Mouse)

Use

Lentiviral

Tags

pPHluorin

Expression

Mammalian

Mutation

The codon were optimized for better expression

Promoter

CMV

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2792

Plasmid

#143193

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF282 (ZNF282 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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paavCAG-Jx

Plasmid

#51905

Purpose

Cre-dependent On/Off aav vector

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Neo

Plasmid

#85139

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBSDONR P4r-P2

Plasmid

#121527

Purpose

Gateway Entry Vector for Multisite Gateway Cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR V7

Plasmid

#86856

Purpose

Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

Depositor

Marco Marcelli, Michael Mancini

Article

Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

Insert

Androgen receptor (AR) (AR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Alternative splice variant 7 (alteration/deletion…

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCHARGE3

Plasmid

#24658

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

Turbo

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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negMaLionB

Plasmid

#113911

Purpose

negative control for Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Article

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

YFP

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9-CR4

Plasmid

#62655

Purpose

Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

Cas9 nuclease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CAPN2

Plasmid

#60942

Purpose

Expresses calpain2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain 2 (CAPN2 Human)

Use

Tags

3xflag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pXG260

Plasmid

#141282

Purpose

CMV:EGFP (as control for pXG237)

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Use

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pVoPo-GFP

Plasmid

#187840

Purpose

Constitutive GFP-expression vector for Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PARP2A

Plasmid

#42450

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PARP2A (PARP2 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pGJJ001

Plasmid

#183619

Purpose

bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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