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Plasmid#89923PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only
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pECT1B
Plasmid#75447PurposePlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1B
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1D
Plasmid#75448PurposePlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1D
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1E
Plasmid#75449PurposePlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1E
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL
Plasmid#64171Purposeharbours marker genes HIS3 and LEU2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLK
Plasmid#64186Purposeharbours marker genes LEU2 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-X4
Plasmid#70140PurposeYeast chr. integration vector with DS3 selection markerDepositorInsertDS3
UseTagsExpressionYeastMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-PqrA
Plasmid#51195DepositorInsertPqrA
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-Venus-cdc25C5
Plasmid#40969DepositorInsertcdc25C5 (CDC25C Human)
UseIn vitro transcriptionTagsVenusExpressionMutationPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNJB99
Plasmid#51521PurposeGateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequenceDepositorInsertZif268:FokI
UseGateway entry vectorTagsNLSExpressionMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-1ALigN
Plasmid#20024DepositorInsert1A Domain of DNA Ligase N
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) A259C
Plasmid#111329PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A259CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) C256A
Plasmid#111331PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site C256APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/tdTomato-nat
Plasmid#120326PurposeBacterial tdTomato labelingDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterproDAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMOD3-mClover-Sh_ble
Plasmid#120329PurposeBacterial mClover promoter trapDepositorInsertmClover
UseTagsExpressionBacterialMutationD103N (see comment below)Promoter(None)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pAKW.119-R5-TEV-SUMO-His
Plasmid#154050PurposeExpresses R5 peptide in E. coliDepositorInsertSilaffin R5 peptide
UseTagsSUMO and His tagExpressionBacterialMutationPromoterT5LacOAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic PancACe
Plasmid#215708PurposeExpresses acetyl-CoA biosensor in the cytoplasmDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial PancACe
Plasmid#215710PurposeExpresses acetyl-CoA biosensor in the mitochondriaDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoSNAP
Plasmid#136623PurposePlasmid encoding outer mitochondrial localization SNAP-TagDepositorInsertNTOM20-SNAP-Tag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only