pHL
(Plasmid
#64171)
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Purpose(Empty Backbone) harbours marker genes HIS3 and LEU2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHLUM (version 2)
- Backbone size (bp) 9905
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Modifications to backboneremoved URA3 and MET17
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersLEU2, HIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL was a gift from Markus Ralser (Addgene plasmid # 64171 ; http://n2t.net/addgene:64171 ; RRID:Addgene_64171) -
For your References section:
Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities. Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M. F1000Res. 2016 Sep 20;5:2351. eCollection 2016. 10.12688/f1000research.9606.1 PubMed 27830062