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Plasmid#45568DepositorInsertTRIM28 (TRIM28 Human)
UseTagsEGFPExpressionMammalianMutationG34S mutation in EGFPPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKH3-TRIM28
Plasmid#45569DepositorInsertTRIM28 (TRIM28 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSP1830
Plasmid#70708PurposeHuman expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAGDepositorInsertmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationE782K, N968K and R1015H in SaCas9PromoterCAGAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-TRIM28
Plasmid#45570DepositorInsertTRIM28 (TRIM28 Human)
UseTagsGSTExpressionBacterialMutationPromoterPtacAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFlox_N-Mau2_Neo_FKBPF36V_mScarlet
Plasmid#163881PurposeAcute Depletion of Mau2-mScarletDepositorInsertMau2 (Mau2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84240PurposeExpresses GA-inducible VPR-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP2292
Plasmid#70707PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFAB3833
Plasmid#47839PurposeBIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.DepositorInsertpromoter 8 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3737
Plasmid#47838PurposeBIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.DepositorInsertpromoter 7 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1
Plasmid#22024PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseTags6X HisExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct4
Plasmid#203789PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorInsertPou5f1 (Pou5f1 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1
Plasmid#22007PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseTags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
p35u4
Plasmid#174786PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid systemDepositorInsertMS2 coat protein
UseTagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFAB3677
Plasmid#47833PurposeBIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.DepositorInsertpromoter 2 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pScalps_puro_mIL-2Rα
Plasmid#59917PurposeExpresses mouse interleukin-2 receptor alpha chainDepositorInsertinterleukin 2 receptor alpha (Il2ra Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFAB3689
Plasmid#47834PurposeBIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.DepositorInsertpromoter 3 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSP2253
Plasmid#70704PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFAB3893
Plasmid#47844PurposeBIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.DepositorInsertpromoter 13 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1
Plasmid#22008PurposeMammalian expression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsmVenusExpressionMammalianMutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct6
Plasmid#203790PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorInsertPou3f1 (Pou3f1 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only