pCAG eGFP-Oct4
(Plasmid
#203789)
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PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAG
- Backbone size w/o insert (bp) 5069
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePou5f1
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Alt name4-Oct
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SpeciesM. musculus (mouse)
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Insert Size (bp)1788
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Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
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Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG eGFP-Oct4 was a gift from Thorsten Wohland (Addgene plasmid # 203789 ; http://n2t.net/addgene:203789 ; RRID:Addgene_203789) -
For your References section:
Selective influence of Sox2 on POU transcription factor binding in embryonic and neural stem cells. Mistri TK, Devasia AG, Chu LT, Ng WP, Halbritter F, Colby D, Martynoga B, Tomlinson SR, Chambers I, Robson P, Wohland T. EMBO Rep. 2015 Sep;16(9):1177-91. doi: 10.15252/embr.201540467. Epub 2015 Aug 11. 10.15252/embr.201540467 PubMed 26265007
