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Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorInsertCD63-mScarlet (CD63 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6a
Plasmid#215819PurposeExpress CBE6a (with SpCas9) in mammalian cellsDepositorInsertCBE6a-SpCas9-2xUGI (cas9 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-SABR-Backbone
Plasmid#119050Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
GW1-Mito-pHRed
Plasmid#31474DepositorInsertCox8-tagged pHRed
UseTags4xCox8ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-2A-GFP-noITR
Plasmid#118415PurposeCas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expressionDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM3
Plasmid#179957PurposeExpression vector for human IFITM3.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sp_PE
Plasmid#212038PurposeBacterial expression of the PE2 proteins for genome editing in E. coliDepositorInsertPE2
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2
Plasmid#193851PurposeE1b promoter for transgenic enhancer assays in zebrafishDepositorInsertEnhanced Green Fluorescent Protein
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TAG-ERP2
Plasmid#80479PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
bact2-mKate2-P2A-TurboID-dVHH[GFP]
Plasmid#163857PurposeZebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBHDepositorInsertTurboID-VHH[EGFP]
UseZebrafish expressionTagsmKate2-P2A-TurboIDExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs ABCDE>A
Plasmid#83318Purposehuman DNA-PKcs/ABCDE>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation6 phosphorylation sites in the ABCDE cluster subs…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a-Cas9-2XNES-2ERT2
Plasmid#120551PurposeExpress Cas9, 2xNES and 2 tandem ERT2 in mammalian cellsDepositorInsertCas9, NES and ERT2
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS MCK promoter
Plasmid#12528DepositorInsertMCK promoter (Ckm Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
UseTags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHybrid-Electra2
Plasmid#179475PurposeExpresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cellsDepositorInsertsElectra2
mScarlet-I
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHyo245
Plasmid#173147PurposeExpresses pheS_A294G by dual T7 promoter in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29
Plasmid#172972PurposeLow copy number cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-hIKAROS-IRES-GFP
Plasmid#74046Purposeretroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1DepositorInserthuman Ikaros (IKZF1) (IKZF1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PARP1
Plasmid#211578PurposeCMV driven PARP1-eGFP expressing construct with IRES-Neomycin selectionDepositorInsertPARP1 (PARP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 D2335A/N2340K
Plasmid#199575PurposeExpresses FLAG-tagged kinase dead SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationD2335A and N2340KPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PMP34-mCherry-EGFP-Tet-On
Plasmid#109017PurposeTet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)DepositorInsertPMP34 (SLC25A17 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Rac1-2G
Plasmid#66110PurposeSecond generation Rac1 FRET biosensorDepositorInsertSecond generation Rac1 FRET biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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SUV420H2
Plasmid#32864PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSUV420H2 (KMT5C Human)
UseTagsHis tag (with TEV protease)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL6-H2B-iRFP720-puro
Plasmid#189992PurposeLentivirial plasmid for nuclear iRFP-720 expression with puromycin resistanceDepositorInsertHuman histone H2B
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1
Plasmid#131725PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-mScarlet-WPRE
Plasmid#99280PurposeCan be used to generate AAV virus that will express mScarlet in the presence of CreDepositorInsertmScarlet
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-EGFP-GPI
Plasmid#65296Purposesynchronize trafficking of EGFP-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYZ301
Plasmid#102690PurposeS. pombe gRNA entry vector with Cas9 - Sp lys9 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKBAD2
Plasmid#15335DepositorInsertDnaE C-intein
UseTagsGB1ExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
p40PX-EGFP
Plasmid#19010DepositorInsertp40phox (NCF4 Human)
UseTagsEGFPExpressionMammalianMutationaa1-148 (PX domains)PromoterCMVAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-biPAC
Plasmid#169127PurposeAAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPACDepositorInsertmKate2-biPAC
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationcodon optimized to mousePromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSOD1-FLAG
Plasmid#182922PurposeMammalian expression plasmid for human WT SOD1DepositorInsert[Cu-Zn] Superoxide dismutase 1 (SOD1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gamillus/ pcDNA3
Plasmid#124837Purposemammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent proteinDepositorInsertGamillus
UseTagsNo tagExpressionMammalianMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
DRD2-DuET
Plasmid#213228PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertDRD2 (DRD2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-C
Plasmid#118369PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Degron-KI SF3B1 donor vector
Plasmid#65622PurposeDonor vector to generate Degron-KI DD-SF3B1DepositorInsertSF3B1 Degron-KI left and right HR arms
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0056 - LwCas13a-msfGFP-NES
Plasmid#105815PurposeExpresses active LwaCas13a-NES for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNES and msfGFP-NES-3xHAExpressionMammalianMutationPromoterEF-1aAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEvol-NnSULT1C1-cysDNCQ
Plasmid#188983Purposebiosynthesize sulfotyrosine in E. coli.DepositorInsertsNnSULT1C1
cysD
cysN
cysC
cysQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp3-myc
Plasmid#11813DepositorInsertCaspase 3 (CASP3 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTol2_LSEC:Cas9green; erbb2_gRNA171
Plasmid#199338PurposepDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNADepositorInsertsLSEC
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-ASAP4e-Kv-WPRE
Plasmid#201031PurposeExpresses somatically enriched ASAP4e in neurons, can be used to package AAVDepositorInsertASAP4e-Kv
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-DAP5-3XFLAG
Plasmid#127331PurposeExpress 3xFLAG tagged DAP5 protein from AUG start codonDepositorInsertDAP5 (EIF4G2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Degron-KI p53 donor vector
Plasmid#65619PurposeDonor vector to generate Degron-KI DD-p53DepositorInsertp53 Degron-KI left and right HR arms
UseTagsExpressionMutationPromoterAvailable sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
R777-E245 Hs.RASGRP2
Plasmid#70529PurposeGateway ORF clone of human RASGRP2 [NM_153819.1] with stop codon (for native or N-terminal fusions)DepositorInsertRASGRP2 (RASGRP2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC2-dual-dCas9VP48-sgExpression
Plasmid#48236PurposeDual expression construct expressing both dCas9VP48 and sgRNA from separate promotersDepositorInsertdCas9VP48
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only