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Plasmid#167689PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chAcK3RS(IPYE)
Plasmid#104069PurposeExpression plasmid encoding chAcK3RS(IPYE) and pylTDepositorInsertPlpp chAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG030 (TerY-P)
Plasmid#157908PurposeExpresses the TerY-P defense systemDepositorInsertTerY-P triad (vWA + kinase + phosphatase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG029 (mzaABCDE)
Plasmid#157907PurposeExpresses the mzaABCDE defense systemDepositorInsertmzaABCDE (5-gene system containing a MutL homolog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEB31
Plasmid#31224DepositorInsertccdB and Cm resistance gene
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSF463
Plasmid#167671PurposeIntermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG034 (restriction-like)
Plasmid#157912PurposeExpresses a restriction-like defense systemDepositorInsert4-gene restriction-like system
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG031 (upx)
Plasmid#157909PurposeExpresses the upx defense systemDepositorInsertupx (putative PD-(D/E)xK family phosphodiesterase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-MYC
Plasmid#101765PurposeGateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion proteinDepositorTypeEmpty backboneUseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG002 (BREX type I)
Plasmid#157880PurposeExpresses the BREX type I defense systemDepositorInsertBREX type I
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPclpBAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLG025 (DUF4297 + HerA)
Plasmid#157903PurposeExpresses the DUF4297 + HerA defense systemDepositorInsertDUF4297 + HerA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG022 (dsr1)
Plasmid#157900PurposeExpresses the dsr1 defense systemDepositorInsertdsr1 (SIR2-DUF4020)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG024 (SIR2 + HerA)
Plasmid#157902PurposeExpresses the SIR2 + HerA defense systemDepositorInsertSIR2 + HerA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG033 (ietAS)
Plasmid#157911PurposeExpresses the ietAS defense systemDepositorInsertietAS (ATPase + serine protease)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG015 (RADAR)
Plasmid#157893PurposeExpresses the RADAR defense systemDepositorInsertRADAR subtype containing auxillary gene rdrD
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG016 (ApeA)
Plasmid#157894PurposeExpresses the ApeA defense systemDepositorInsertApeA (putative HEPN RNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only