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Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBIS
Plasmid#35150DepositorInsertAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLacUV5,Available sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMW#2
Plasmid#13349DepositorInsertHIS3 reporter
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-mSc
Plasmid#187842PurposeConstitutive mScarlet-I-expression vector for Fusobacterium nucleatumDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG002 (BREX type I)
Plasmid#157880PurposeExpresses the BREX type I defense systemDepositorInsertBREX type I
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
CpxR-GFP
Plasmid#220150PurposeTo track the natural CpxR promoter's transcriptional dynamicsDepositorInsertCpxR promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMW#3
Plasmid#13350DepositorInsertLacZ reporter
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmPylRS(IPYE)
Plasmid#104073PurposeExpression plasmid encoding MmPylRS(IPYE) and pylTDepositorInsertPlpp MmPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR291
Plasmid#79555PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.DepositorInsertsGFPmut3b
mTagBFP
mRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWS3799-gRNA-Csy4-template
Plasmid#182827PurposeSpCas9 gRNA-Csy4 templateDepositorInsertSpCas9 gRNA scaffold + Csy4 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-crRNA-Cm
Plasmid#158711PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInserteforCP (for crRNA cloning)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterTetOAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi-ispA/dxs
Plasmid#178302PurposeProduces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.DepositorInsertsispA
dxs
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-H3
Plasmid#109381PurposeArabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCS104
Plasmid#209357PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only