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Showing: 3581 - 3600 of 28583 results

  5. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  8. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  12. pAAV-HDC-DIO-GFP-shRNA.scramble

    Plasmid
    #184331
    Purpose
    Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
    Use
    AAV, Cre/Lox, Mouse Targeting, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    histidine decarboxylase promoter fragment that g…
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    184331-plasmid-map-sequence-id-365459

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  13. pAAV-flex-GFP-shRNA-GluN1

    Plasmid
    #182502
    Purpose
    Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182502-plasmid-map-sequence-id-355962

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  14. pAAV-iCre-2A-cherry

    Plasmid
    #182246
    Purpose
    Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182246-plasmid-map-sequence-id-355958

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  16. Anti-VGlut3 [N34/34R-2b]

    Plasmid
    #219476
    Purpose
    Mammalian Expression Plasmid of anti-VGlut3 neurotransmitter transporter (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N34/34.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-VGlut3 neurotransmitter transporter (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc17a8 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219476-plasmid-map-sequence-id-430663

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  17. Anti-VGlut1 [N28/9R-rat IgG2a]

    Plasmid
    #222184
    Purpose
    Mammalian Expression Plasmid of anti-VGlut1 neurotransmitter transporter (Rat). Derived from hybridoma N28/9.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-VGlut1 neurotransmitter transporter (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc17a6 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222184-plasmid-map-sequence-id-437282

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  18. Anti-NPY2R [L119/106R-2b]

    Plasmid
    #211594
    Purpose
    Mammalian Expression Plasmid of anti-NPY2R/Neuropeptide Y receptor type 2 (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma L119/106.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) recombinant mouse monoclonal antibody. (NPY2R Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211594-plasmid-map-sequence-id-418271

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  19. Anti-VGlut2 [N29/29R-1]

    Plasmid
    #206707
    Purpose
    Mammalian Expression Plasmid of anti-VGlut2 neurotransmitter transporter (Rat). Derived from hybridoma N29/29-1.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-VGlut2 neurotransmitter transporter (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc17a6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206707-plasmid-map-sequence-id-414160

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  20. Anti-VGlut1 [N28/9R-1]

    Plasmid
    #206706
    Purpose
    Mammalian Expression Plasmid of anti-VGlut1 neurotransmitter transporter (Rat). Derived from hybridoma N28/9-1.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-VGlut1 neurotransmitter transporter (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc17a7 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206706-plasmid-map-sequence-id-414159

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Showing: 3581 - 3600 of 28583 results