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pcDNA3-pri-let-7d

Plasmid

#51379

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7d (MIRLET7D Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SypHluorin 1x (S1x)

Plasmid

#37003

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin (Syp Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

One copy of pHluorin inserted at site 184-185

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-KRasCT

Plasmid

#99017

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

mCherry KRas CT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Mis12-targted Aurora B FRET sensor

Plasmid

#45231

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NM23-H1

Plasmid

#10956

Depositor

Patricia Steeg

Article

Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

Insert

NM23 (NME1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lis1

Plasmid

#12574

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Lis1 (Pafah1b1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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mSECFP/pcDNA3

Plasmid

#51951

Purpose

mammalian expression of mSECFP, a monomeric super enhanced cyan fluorescent protein

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

mSECFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pnGFP

Plasmid

#64913

Purpose

Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Insert

pnGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_27B3_hFc

Plasmid

#199618

Purpose

Encodes DARPin-hFc 27B3 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc 27B3

Use

Tags

hFc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-pri-let-7e

Plasmid

#51380

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7e (MIRLET7E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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HfACR1_EYFP_pcDNA3.1

Plasmid

#161026

Purpose

Expresses strongly red-shifted HfACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

HfACR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+):RGS4

Plasmid

#31157

Depositor

Henry Lester

Article

Doupnik et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

Regulator of G Protein Signaling 4 (Rgs4 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4-V5-LUC (w468-1)

Plasmid

#19135

Purpose

Gateway entry vector, V5-Luciferase

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Entry vector

Tags

V5

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 epsilon wt

Plasmid

#11942

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pBAD-hsGFP

Plasmid

#64911

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Use

Tags

His Tag

Expression

Bacterial

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

araBAD

Available since

May 20, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4-GST 6P-1 (w487-1)

Plasmid

#17741

Purpose

Gateway entry vector for N-term GST fusion, frame 6P-1

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

GST

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-NS

Plasmid

#75341

Purpose

Multisite gateway entry clone for adding N-terminal fusions of EGFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge