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Addgene

pcDNA3-pnGFP
(Plasmid #64913)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64913 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5968
  • Total vector size (bp) 6748
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pnGFP
  • Species
    Synthetic
  • Insert Size (bp)
    780
  • Mutation
    Chromophore tyrosine of the protein mutated to TAG amber codon
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please cite Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-pnGFP was a gift from Huiwang Ai (Addgene plasmid # 64913 ; http://n2t.net/addgene:64913 ; RRID:Addgene_64913)
  • For your References section:

    Genetically encoded fluorescent probe for the selective detection of peroxynitrite. Chen ZJ, Ren W, Wright QE, Ai HW. J Am Chem Soc. 2013 Oct 9;135(40):14940-3. doi: 10.1021/ja408011q. Epub 2013 Sep 26. 10.1021/ja408011q PubMed 24059533