pcDNA3-pnGFP
(Plasmid
#64913)
-
PurposeEncodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 64913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5968
- Total vector size (bp) 6748
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH10B
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepnGFP
-
SpeciesSynthetic
-
Insert Size (bp)780
-
MutationChromophore tyrosine of the protein mutated to TAG amber codon
- Promoter CMV
-
Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pCMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please cite Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3-pnGFP was a gift from Huiwang Ai (Addgene plasmid # 64913 ; http://n2t.net/addgene:64913 ; RRID:Addgene_64913) -
For your References section:
Genetically encoded fluorescent probe for the selective detection of peroxynitrite. Chen ZJ, Ren W, Wright QE, Ai HW. J Am Chem Soc. 2013 Oct 9;135(40):14940-3. doi: 10.1021/ja408011q. Epub 2013 Sep 26. 10.1021/ja408011q PubMed 24059533