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bdSUMO-5.3-GFP-HiBit

Plasmid

#171014

Purpose

For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-5.3-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK2 3'UTR KGA

Plasmid

#110402

Purpose

Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 800

Plasmid

#131147

Purpose

To investigate activity of a 800 bp region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 2.6

Plasmid

#131151

Purpose

To investigate activity of a 2.6 kb region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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hsa-cdk2 3'UTR sicheck2

Plasmid

#121125

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-cdk2 3'UTR (CDK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 T2P 500

Plasmid

#131150

Purpose

To investigate activity of a 500 bp region upstream of mouse Trib2.

Depositor

Karen Keeshan

Article

Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

Insert

-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hARPC2

Plasmid

#112549

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Li et al Sci Rep. 2017 Apr 19;7(1):915. doi: 10.1

Insert

ARPC2 (ARPC2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom2.5

Plasmid

#87351

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

2.5 kbp proximal promoter of human MCT2 (nested d…

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom1.1

Plasmid

#87352

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

1.1 kbp proximal promoter of human MCT2 (nested d…

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom3.2

Plasmid

#87349

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

3.2 kbp proximal promoter of MCT2 (nested deletio…

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0-GL-2-3 perfect copies

Plasmid

#82441

Purpose

Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Use

Tags

Expression

Mutation

The complementary region for GL2 siRNA control wa…

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(WT)

Plasmid

#86576

Purpose

Expresses cleavable human full-length Akt1(WT)

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-PIM2mut3

Plasmid

#53742

Purpose

promoter region of PIM2 lacking the 2 forkhead binding sites

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Tags

Expression

Mutation

deletion of 2 forkhead binding sites (bp# 395-497…

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant1

Plasmid

#45384

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI1 promoter -732/-721 mutated at -732/-731

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutated -732/-731 from ag to tc

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
psiCheck-DNAJA43'UTR

Plasmid

#41847

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

DNAJA4 3'UTR (DNAJA4 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
CS2 luc MutA

Plasmid

#13498

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

Fstl1 (Fstl1 Mouse)

Use

Luciferase

Tags

Expression

Mutation

contains 3 tandem copies of the following sequenc…

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
CS2 luc MutB

Plasmid

#13499

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

Fstl1 (Fstl1 Mouse)

Use

Luciferase

Tags

Expression

Mutation

contains 3 tandem copies of the following sequenc…

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Gluc-LHCGR

Plasmid

#221781

Purpose

Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

Depositor

Adolfo Rivero-Muller

Article

Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

Insert

LHCGR (LHCGR Human)

Use

Tags

Gaussia Luciferase and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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